| 新生小鼠小肠Cajal间质细胞增殖的实验研究 |
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| 引用本文： | 于彬,韩鹃,梅峰.新生小鼠小肠Cajal间质细胞增殖的实验研究[J].中国局解手术学杂志,2009,18(3). |
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| 作者姓名： | 于彬 韩鹃 梅峰 |
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| 作者单位： | 第三军医大学基础部组织学与胚胎学教研室,重庆,400038
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| 基金项目： | 国家自然科学基金，重庆市自然科学基金 |
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| 摘 要： | 目的 探讨在新生小鼠小肠Cajal间质细胞(interstitial cells of Cajal,ICC)的发育过程中是否出现增殖.方法 采用新生2 d(P2d)、14 d(P14d)和24 d(P24d)的小鼠小肠,应用BrdU腹腔注射,24 h后取材,Kit和BrdU免疫荧光染色.结果 P2d小鼠小肠,可见大量Kit/BrdU双重标记阳性细胞,这些细胞的形态与成熟ICC基本相似,这些细胞在P14d时减少,而在P24d消失.统计学分析发现,不同时间点之间Kit/BrdU双标阳性细胞数量存在显著差别(P＜0.05).结论 在小肠生后发育过程中,出现了ICC增殖,而这种增殖在发育过程中逐渐减弱,最后在成年前消失.
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| 关 键 词： | Cajal间质细胞 增殖 Kit蛋白 |
A study on interstitial cells of Cajal proliferation in murine small intestine during postnatal peroid |
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| YU Bin,HAN Juan,MEI Feng.A study on interstitial cells of Cajal proliferation in murine small intestine during postnatal peroid[J].Journal of Regional Anatomy and Operative Surgery,2009,18(3). |
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| Authors: | YU Bin HAN Juan MEI Feng |
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| Institution: | Department of Histology and Embryology;Third Military Medical University;Chongqing 400038;China |
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| Abstract: | Objective To investigate the appearance of interstitial cells of Cajal proliferation during postnatal development.Methods Postnatal mice aged 2 days(P2d),14 days(P14d) and 24 days(P24d) were used,and intraperitoneally injection of BrdU was carried out 24 hours before sacrifice.Immunohistochemcal staining with either Kit or BrdU antibody was performed to reveal the proliferative ICC.Results Kit/BrdU double labeling cells were found out in the small intestine of 2-days old mice,and these cells were similar wi... |
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| Keywords: | BrdU |
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