生长抑素2型受体mRNA在人胃上皮永生细胞及肿瘤细胞株？…

目的 了解多种癌细胞上是否较广泛表达生长抑素２型受体,同时了解ｐ５３０突变与否对生长抑素２型受体的表达有无影响。方法 用ＲＴ－ＰＣＲ和克隆的人生长抑素２型受体ｃＤＮＡ为探针检测人胃上皮永生细胞、人胃癌细胞、胰腺癌细胞、结肠癌细胞、膀胱癌细胞共１０株细胞上该受体ｍＲＮＡ的表达。结果 发现除１株胰腺癌细胞外,其余９个细胞株均表达生长抑素２型受体。一株胃癌细胞分别转染了野生型和突变型的ｐ５３基因,也同样

Somatostatin receptor subtype 2 mRNA expression in human gastric immortalized cell line and tumor cell lines

OBJECTIVE: Inhibition of somatostatin on cell proliferation via somatostatin receptor subtype 2 (SS-R2), suggested this receptor subtype may be involved in tumor growth. Present study is to demonstrate whether SS-R2 was expressed in tumor cell lines extensively as well as in human gastric epithelia immortalized cell line. Meanwhile, the effect of p53 mutation on SS-R2 expression in gastric cancer cell line was also elucidated. METHODS: Full-length human somatostatin receptor subtype 2 cDNA was cloned by RT-PCR. Using cDNA as the probe and RT-PCR, expression of mRNA for SS-R2 in 9 cancer cell lines and human gastric immortalized cell line was detected. RESULTS: Data revealed that most cancer cell lines but a pancreatic cancer cell line expressed SS-R2 mRNA. CONCLUSION: Regardless whether p53 mutation or not in human gastric cancer cell lines, SS-R2 mRNA was transcribed.