sGC和TPO测定鉴别原发性血小板减少性紫癜和再生障碍性贫血的价值

目的探讨可溶性血小板糖盏蛋白（sGC）和血小板生成素（TPO）测定在特发性血小板减少性紫癜（ITP）和再生障碍性贫血（AA）鉴别诊断中的临床意义。方法用建立的sGC免疫放射分析法（mMA）和酶联免疫吸附测定（ELISA）法分别测83例ITP、47例AA患者及50名健康人血浆中的sGC和血清TPO水平。用方差分析Q值检验进行统计学分析，P〈0．05为差异有统计学意义。结果ITP组患者血浆sGC水平为（1024．35±232．47）μg／L，健康组为（712．50±177．11）μg／L，两者差异有统计学意义（Q=4．11，P〈0．05），而AA组患者血浆sGC为（543．56±141．28）μg／L，与健康对照组比较，差异也有统计学意义（Q=3．45，P〈0．05）；ITP组患者血清TPO为（90．32±39．43）ng／L，健康对照组为（70．29±25．16）ng／L，差异无统计学意义（Q=2．68，P〉0．05），而AA组血清TPO为（857．43±228．43）ng／L，高于ITP组和健康对照组（Q=24．45和18．25，P均〈0．01），差异有统计学意义。结论血浆sGC和血清TPO的测定有助于ITP和AA鉴别诊断及其病理机制研究。

Clinical significance of detecting soluble glycocalicin and thrombopoietin in the differential diagnosis of idiopathic thrombocytopenic purpura and aplastic anemia

Objective To investigate the clinical significance of detecting soluble platelet glycocal-icin (sGC) and thrombopoietin (TPO) in the differential diagnosis of idiopathic thrombocytopenic purpura (ITP) and aplastic anemia (AA). Methods Plasma sGC and serum TPO in 83 patients with ITP, 47 pa-tients with AA and 50 normal individuals were detected by iminunoradiometric assay (IRMA) and enzyme-linked immunosorbent assay (ELISA), respectively. Statistical analysis was performed using Q test and P value of < 0.05 was considered statistically significant. Results Plasma sGC in ITP group was significant-ly higher than that of normal group: (1024.35±232.47)μg/L vs (712.50±177.11)μg/L, and it was considered statistically significant (Q=4.11, P<0.05). But plasma sGC in AA group was significantly lower than that of normal group: (543.56±141.28)μg/L vs (712.50±177.11)μg/L, which was statis-tically significant (Q=3.45, P <0.05). TPO level in ITP group was in the same range as that in normal controls: (90.32±39.43) ng/L vs (70.29±25.16) ng/L, which was statistically insignificant (Q=2.68, P > 0.05). But serum TPO level in AA group was significantly higher than that in ITP and normal groups: (857.43±228.43) ng/L vs (90.32±39.43) ng/L and (70.29±25.16) ng/L, and they were considered statistically significant (Q=24.45 and 18.25, both P < 0.01). Conclusion Detecting plasma sGC and serum TPO might be helpful for differentiating ITP and AA and for understanding the pathophysiol-ogy of thrombocytopenia.