高效液相色谱法测定小儿贝诺酯维生素B1颗粒的含量

目的：建立高效液相色谱法测定小儿贝诺酯维生素B，颗粒中贝诺酯和维生素B。的含量。方法：采用Shim-packVP．ODS柱（150mm×4．6mm，5μm），流动相为甲醇-0．005mol·L-1庚烷磺酸钠溶液（50：50）（用磷酸调节pH至3．5），流速1ml·min-1，检测波长260am，柱温30℃。结果：贝诺酯在18．052～162．468mg·L-1的范围内线性关系良好（r=0．9999，n=5），平均回收率为100．2％，RSD=1．2％（n=6）；维生素B1在5．956～53．604mg·L-1的范围内线性关系良好（r=0．9999，n=5），平均回收率为99．3％，RSD：2．1％（n=6）。结论：本方法简便，准确，专属性好。

Determination of Pediatric Benorilate and Vitamin B1 Granules by HPLC

Objective： To establish an HPLC method for the determination of benorilate and vitamin Bl in pediatric benorilate and vitamin B1 granules. Method： The Shim-pack VP-ODS（ 150 mm ×4.6 mm,5μm）column was used. The mobile phase consisted of methanol and 0. 005 mol-L-1 heptane sulfonic acid sodium solution （50： 50, pH adjusted to 3.5 with phosphoric acid）. The flow rate was 1.0 ml. min - 1 with a detector wavelength at 260 mn and column temperature was 30 ~C. Result： The linear range of benorilate was 18. 052-162. 468 mg.L-1 （r =0. 999 9 ,n =5） ,the average recovery was 100. 2% （RSD = 1.2% ,n =6） ;The linear range of vitamin B~ was 5. 956-53. 604 mg.L-1 （ r =0. 999 9 ,n = 5 ） ,the average recovery was 99.3% （RSD =2.1% ,n =6）. Conclusion： This method is simple, accurate and suitable.