cAMP对人骨髓瘤细胞的IL-6信号转导功能及细胞增殖的抑制作用

目的 研究ＰＫＡ途径激活（或胞内ｃＡＭＰ水平升高）后对人骨髓瘤细胞的ＩＬ－６信号转导功能及细胞生长的影响。方法 采用ＰＫＡ途径激动剂Ｆｏｓｋｏｌｉｎ（ＦＫ）和ｃＡＭＰ类似物８－Ｂｒ－ｃＡＭＰ刺激人骨髓瘤细胞骨－Ｓｋｏ－００７，分别通过ＭＴＴ方法和凝胶阻滞电泳（ｅｌｅｃｔｒｏｐｈｏｒｅｔｉｃ ｍｏｂｉｌｉｔｙ ｓｈｉｆｔ ａｓｓａｙ，ＥＭＳＡ）方法检测ＦＫ和８－Ｂｒ－ｃＡＭＰ对Ｓｋｏ细胞的生长及其Ｉ

cAMP inhibits the proliferation and IL-6 signal transduction of multiple myeloma cell

Objective To investigate the effect of PKA pathway activation (or increasing level of intracellular concentration of cAMP) on the growth and IL 6 signal transduction of the multiple myeloma (MM) cells. Methods Human multiple myeloma (MM) cell line Sko 007 were stimulated with the angonist of PKA forskolin(FK) or the analogue of cAMP 8 Br cAMP, the effect of FK and 8 Br cAMP on cell proliferation and IL 6 signal transduction of Sko 007 cells were exhibited by MTT and electrophoretic mobility shift assay(EMSA). Results FK and 8 Br cAMP inhibited the proliferation and Jak/STAT pathway activation of Sko 007 cells, but had no regulatory effect on Ras/NF IL 6 pathway. Conclusion 1.Jak/STAT pathway activation by IL 6 in MM cell line Sko 007 can be regulated by cAMP. 2.The inhibitory effect on the proliferation of Sko cells mediated by the activation of PKA pathway is temporally related to the inhibition of JAK/STAT pathway activation by IL 6.