| 结核分枝杆菌免疫层析和荧光PCR联合检测方法的应用 |
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| 引用本文： | 王佃鹏,;董瑞玲,;武学成,;朱玉兰,;张艳芳,;李智民,;惠长野,;杨祥丽.结核分枝杆菌免疫层析和荧光PCR联合检测方法的应用[J].热带医学杂志,2014(8):1033-1034. |
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| 作者姓名： | 王佃鹏 ;董瑞玲 ;武学成 ;朱玉兰 ;张艳芳 ;李智民 ;惠长野 ;杨祥丽 |
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| 作者单位： | [1]深圳市职业病防治院,广东深圳518001; [2]深圳国际旅行卫生保健中心,广东深圳518033; [3]深圳市龙华新区慢性病防治中心,广东深圳518006 |
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| 基金项目： | 国家质检总局科技计划项目（2013IK236）; 职业病国家临床重点专科建设项目（WY2011873）; 深圳市宝安区科技计划项目（2012226） |
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| 摘 要： | 目的探讨一种基于免疫层析法和荧光PCR DNA定性检测技术的肺结核多重诊断模式。方法以208例疑似结核患者为研究对象,采用免疫层析法检测其血清特异性抗结核抗体,同时采用荧光PCR方法检测其痰液中结核分枝杆菌DNA,并将两种方法的检测结果进行比较。结果免疫层析法检测结果显示,16份血清标本呈阳性,阳性率为7.7%;荧光PCR方法检测结果显示,14份痰液标本呈阳性,阳性率为6.7%。两种检测方法协同诊断率为100.0%。结论结核分枝杆菌免疫层析法和荧光PCR法相结合的实验室诊断模式有助于肺结核的快速诊断和传染风险性评估。
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| 关 键 词： | 结核分枝杆菌 免疫层析法 荧光PCR法 |
A study of combined detection method for Mycobacterium tuberculosis with immune chromatography and fluorescence PCR |
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| Institution: | WANG Dian-peng, DONG Rui-ling, WU Xue-cheng, ZHU Yu-la, ZHANG Yan-fang, LI Zhi-ming, HUI Chang-ye, YANG Xiang-li(1. Shenzhen Hospital for Occupational Disease Prevention and Treatment, Guangdong, Shenzhen 518001 ; 2. Shenzhen International Travel Health Care Center, Guangdong, Shenzhen 518033 ; 3. Shenzhen Longhua New Center for Chronic Disease Prevention and Treatment, Guangddng, Shenzhen 518006, China) |
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| Abstract: | Objective To investigate a multi-diagnostic model for Mycobacterium tuberculosis based on immune chromatography and fluorescence PCR DNA qualitative detection technology. Methods 208 samples were detected by immune chromatography for specific anti-TB antibodies of serum and fluorescent PCR method for sputum. Results The 16 serum samples of the test results using immune chromatography were positive, with a positive rate of 7.7%; the 14 sputum samples of the test results using fluorescent PCR were positive, with a positive rate of 6.7%. The Collaborative diagnosis rate of the two detection methods was 100.0%. Conclusion The diagnostic laboratory Combination mode for Mycobacterium tuberculosis using immune chromatography and fluorescence PCR can quickly diagnose and assess the risk of tuberculosis infection. |
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| Keywords: | Mycobacterium tuberculosis immune chromatography fluorescent PCR |
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