基因重组纤溶酶原 Kringle 5 对血管生成抑制作用的研究

目的 研究基因重组纤溶酶原 (Kringle 5, K5) 对小鼠的血管生成的抑制作用。 方法 以体外 培养的小鼠内皮细胞为研究对象, 利用划痕实验和黏附能力测定实验观察重组 K5 对内皮细胞迁移、 黏附 能力的影响; 利用体外血管生成体系、 创伤愈合和鸡胚尿囊膜 ( chicken embryo allantoic membrane, CAM) 血管生成能力实验, 检测 K5 对血管生成作用的影响。 结果 200 nmol / L K5 显著抑制内皮细胞的迁移、 黏 附以及新生血管的生长长度。 并且 K5 在 2 ~ 18 mg / kg 浓度范围内对小鼠创伤愈合具有明显的抑制作用。 此 外, 6、 12 与 25 mg / L K5 显著抑制 CAM 血管生成, 25 mg / L K5 的抑制作用最强。 结论 K5 能明显抑制血 管生成, 为临床抑制血管生成治疗提供理论依据。

Inhibitory Effect of Recombinant Plasminogen Kringle 5 on Neovascu-larization

Objective To investigate the inhibitory effect of recombination plasminogen Kringle 5 (K5) on angiogenesis in mice. Methods Scratch assay and adhesion ability assay were used to determine the effect of recombinant K5 on the migration and adhesion of in vitro cultured mouse endothelial cells. The effect of K5 on angiogenesis was detected by utilizing in vitro angiogenesis system, wound healing and chicken embryo allantoic membrane ( CAM) angiogenesis ability assay. Results K5 (200 nmol / L) markedly inhibited the migration and adhesion of endothelial cells as well as the growth of new blood vessels. K5 also significantly inhibited wound healing in mice at the concentration of 2 to 18 mg / kg. In addition, 6, 12 and 25 mg / L K5 significantly inhibited CAM neovascularization, with the strongest inhibitory effect of K5 at 25 mg / L. Conclusion These results suggest that K5 can significantly inhibit the angiogenesis of vascular endothelial cells, which provides a theoretical basis for clinical treatment of angiogenesis