自体牙-骨移植联合富血小板纤维蛋白影响种植体周围缺损的机制

目的 探究自体牙-骨移植联合富血小板纤维蛋白影响种植体周围缺损的机制。 方法 将 40 只新 西兰大白兔随机分为实验 A、 B、 C 组和空白对照组, 每组 10 只, 均在全身麻醉的状态下通过微创术拔除 上颌前部单颗牙齿, 实验 A 组大白兔牙窝内植入自体牙-骨移植联合富血小板纤维蛋白, 实验 B 组仅植入 自体牙-骨移植, 实验 C 组仅植入富血小板纤维蛋白, 空白对照组则不植入任何材料。 对比分析各组碱性磷 酸酶 ( alkaline phosphatase, ALP ) 活 性、 Ⅰ 型 胶 原、 骨 保 护 素 ( osteoprotegerin, OPG ) 及 其 配 体 (RANKL) 灰度值、 Runx2、 ALP 以及血小板反应蛋白-1 mRNA 表达水平差异。 结果 随着时间的延长, 实 验组 ALP 活性, Ⅰ型胶原和 OPG 灰度值, Runx2、 ALP 和血小板反应蛋白-1 mRNA 表达水平均高于空白对 照组; 实验 A 组上述各指标值高于实验 B 组和 C 组, 差异有统计学意义 (P< 0. 05)。 各组 RANKL 灰度值 虽然随着时间延长增加, 差异有统计学意义 (P< 0. 05), 但在各时间点, 实验组和空白对照组 RANKL 灰 度值差异无统计学意义 (P> 0. 05)。 结论 联用富血小板纤维蛋白增强了自体牙-骨移植过程中对种植体周 围缺损修复的作用。

Effectof Autologous Tooth-bone Grafting Combined with Platelet-rich Fibrin on Peri-implant Defects

Objective To investigate the effect of autologous tooth-bone graft combined with platelet-rich fibrin on peri-implant defects and the underlying mechanism. Methods A total of 40 New Zealand white rabbits were randomly divided into the experimental groups A, B, C and the blank control group. Under the condition of general anesthesia, a minimally invasive surgical procedure was performed to extract the animals’maxillary anterior teeth. The tooth socket of rabbits in the experimental group A were implanted with autologous tooth-bone grafts combined with platelet-rich fibrin. That in experimental group B was only implanted with autogenous tooth-bone grafts. Rabbits in experimental group C experienced only the implantation with platelet-rich fibrin, and no material was implanted in blank control group. The activity of alkaline phosphatase (ALP), the gray values of typeⅠcollagen, osteoprotegerin (OPG) and its ligand (RANKL), the mRNA expression levels of Runx2, ALP and platelet reactive protein-1 were compared and analyzed. Results The ALP activity, the gray values of typeⅠcollagen and OPG, the mRNA expression levels of Runx2, ALPand platelet reactive protein-1 in the experimental groups were higher than those in the blank control group. The values of the above indexes in the experimental group A were significantly higher than those in experimental groups B and C ( P < 0. 05). Although the gray values of RANKL in each group were significantly increased with time (P< 0. 05), there was no significant difference in the gray values of RANKL between the experimental groups and the blank control group at each time point (P > 0. 05). Conclusion The combination of platelet-rich fibrin enhanced the repair of peri-implant defects in the process of autologous tooth-bone transplantation.