运动训练对心理应激大鼠下丘脑-垂体-性腺轴的影响

目的:探讨运动训练对心理应激大鼠下丘脑-垂体-性腺(HPG)轴的影响。方法:将50只SD大鼠随机分为对照组、运动组、急性心理应激组、慢性心理应激组、急性心理应激 运动组、慢性心理应激 运动组。运动大鼠进行10周游泳运动(1次/天,60min/次);心理应激大鼠分别施加急(一次)、慢(后3周)性心理应激。测定各组大鼠下丘脑GnRHmRNA、垂体LHmRNA表达及血清睾酮(T)、皮质醇(C)含量。结果:(1)急、慢性心理应激组大鼠下丘脑GnRH、垂体LHmRNA表达量、血清T水平均显著低于对照组,血清C水平显著高于对照组;慢性心理应激组大鼠体重、睾丸重、睾丸重/体重均显著低于对照组。(2)与对照组相比,运动组大鼠下丘脑GnRH、垂体LH表达及血清T、C均无明显变化。(3)与心理应激组比,慢性心理应激 运动组大鼠下丘脑GnRH、垂体LH表达量以及血清T水平显著升高。结论:心理应激作为一种应激源,可能会引起大鼠HPG轴紊乱;中等负荷运动对提高机体抗应激能力有一定作用。

Effects of Exercise on HPG Axis in Rats with Psychological Stress

Objective To study the effects of exercise on the HPG axis in rats with psychological stress.Methods Fifty SD rats were divided randomly into control group(S0P0),exercise group(P1),acute psychological stress group(S1),chronic psychological stress group(S2),exercise plus acute psychological stress group(S1P1),exerciseand plus chronic psychological stress group(S2P1).Exercise groups underwent Swimming for 10 weeks,acute or chronic psychological stress was induced by Communication Box.The GnRH mRNA in hypothalamus,LH mRNA in pituitary,and T and C in serum were tested.Results(1)Both acute and chronic psychological stress could decrease GnRH mRNA in hypothalamus,LH mRNA in pituitary,and T and inecrease C in serum significantly.Chronic psychological stress decrease body weight,testicle weight and BW/TW markedly.(2)Exercise showed no effects on GnRH mRNA in hypothalamus and LH mRNA in pituitary and T in serum.(3)Exercise increased the GnRH mRNA in hypothalamus,LH mRNA in pituitary,and T in serum significantly in the chronic psychological stress group.Concludsion Psychological stress induced disorder of HPG axis in rat and exercise promoted anti-stress ability.