血管紧张素转换酶基因多态性与高血压微量蛋白尿的关系

为研究血管紧张素转换酶基因插入/缺失（I/D）多态性与高血压微量蛋白尿的关系。应用聚合酶链反应方法扩增50例正常人，50例高血压伴有微量蛋白尿患者和49例高血压不伴有微量蛋白尿患者的白细胞血管紧张素转换酶基因上287bp片段，根据插入（Ⅰ）或/缺失（D）来判断其多态性，用放射免疫法测定所有对象的尿微量白蛋白。结果发现，微量蛋白尿组与健康对照组相比，其D等位基因及DD基因型显著升高。微量蛋白尿组与单纯高血压组相比，其D等位基因及DD基因型显著程式高。单纯高血压组与健康对照组相比，血管紧张素转换酶基因型和等位基因频率无显著性差异。以上提示，血压紧张素转换酶基因多态性与高血压微量蛋白尿有关联性，DD基因型可能与高血压早期肾脏损害有关。

The Relationship between Angiotensin-Converting Enzyme Gene Polymorphism and Microalbuminuria in Patients with Essential Hypertension

Aim To investigate the relationship between angiotensin converting enzyme (ACE) gene polymorphism and microalbuminuria in patients with essential hypertension Methods Polymerase chain reaction (PCR) was used to determine the genotypes for an insertion/deldtion polymorphism of the ACE gene in 50 hypertensive patients with microalbuminuria and 49 hypertensive patients without microalbuminuria and 50 healthy controls. Urine MAlb concentrations were determined by radioimmunoassay. Results There was significant difference in the frequencies of D allele and DD genotype between hypertensive patients with microalbuminuria and healthy controls. The frequencies of D allele and DD genotype was higher in hypertensive patients with microalbuminuria and lower in those without microalbuminuria. However, ACE I/D polymorphism did not differ in hypertensive patients with non microalbuminuria and healthy controls. Conclusion ACE gene polymorphism was closely related with hypertensive microalbuminuria. DD genotype was possibly involved in the renal injury in patients with hypertension.