MTT法检测蝙蝠葛活性成分的抑制肿瘤增殖作用

目的]研究蝙蝠葛活性成分对多种瘤株的抑制肿瘤增殖作用.方法]取体外培养的人宫颈癌Hela细胞株、人肺癌A-549细胞株及人肝癌HepG-2细胞株,给予蝙蝠葛活性成分作用24h,MTT法检测抑制增殖作用.结果]蝙蝠葛活性成分作用于人宫颈癌Hela细胞株时,抑制率最高达到99.9%,对人肺癌A-549细胞株时最高达到99.4%,对人肝癌HepG-2细胞株时最高达到87.8%.结论]蝙蝠葛活性成分对人宫颈癌Hela细胞株、人肺癌A-549细胞株及人肝癌HepG-2细胞株具有明显的抑制增殖作用,抑制率有随着质量浓度增加而升高的趋势.

Detection of anti-tumor proliferation of active components of the Menispermum dauricum by MTT method

OBJECTIVE To study the anti-tumor proliferation of active components of the Menispermum dauricum.METHODSThe cerical cancer Hela cells,lung cancer A-549 cells,and liver HepG-2 cells were cultured in vitro by administrating with active components of the Menispermum dauricum for 24 hours,and the anti-tumor proliferation was detected by MTT method.RESULTSThe inhibition ratio of active components of the menispermum dauricum for the cerical cancer Hela cells,lung cancer A-549 cells and liver HepG-2 cells was more than 99.9%,99.4% and 87.8%,respectively.CONCLUSIONThe active components of the Menispermum dauricum inhibit the proliferative effects for the cerical cancer Hela cells,lung cancer A-549 cells and liver HepG-2 cells in vitro,and the inhibition ratio is increasing along with the dose-dependent manner.