酸性微环境对单核/巨噬细胞分泌血管内皮细胞生长因子及Tca8113细胞杀伤作用的影响

目的通过酸性微环境下单核/巨噬细胞分泌血管内皮细胞生长因子（VEGF）及对舌癌细胞株Tca8113杀伤作用的研究，探讨肿瘤内浸润的单核/巨噬细胞在酸性微环境影响下的功能状态。方法从健康人的外周血中分离单核细胞并通过培养将其转化为单核/巨噬细胞，分别调整培养液的pH值为酸性：6.6、6.8；常规环境：pH值为7.2，将单核/巨噬细胞和舌癌细胞株Tca8113进行混合培养，MTT法检测单核/巨噬细胞对舌癌细胞的杀伤作用；采用ELISA法检测不同微环境下单核/巨噬细胞VEGF的分泌情况。结果在酸性微环境下，单核/巨噬细胞对舌癌细胞株的杀伤作用低于常规环境下（P<0.05）；而分泌VEGF的能力较常规环境下增强（P<0.05）。结论可能正是肿瘤组织局部的酸性微环境改变了单核/巨噬细胞的功能，更多的参与了肿瘤的生长，但其在肿瘤中的具体机制及如何调节单核/巨噬细胞在肿瘤组织中的功能还需进一步的研究。

Experimental study on the anti-tumor effect of monocytes/macrophages against Tca8113 cells and the se

Objective To investigate the anti-tumor effect of monocytes/macrophages against Tca8113 cells and the secretion of vascular endothelial growth factor（VEGF） in acid microenvironment in vitro. Methods Peripheral blood mononuclear cells were extracted from healthy person′ s blood and cultivated to transform into monocytes/ macrophages. The monocytes/macrophages were cultured with Tca8113 in acid microenvironment（pH6.6 and pH6.8） and in normal microenvironment（pH7.2）. The anti-tumor effect of monocytes/macrophages against Tca8113 cells were examined by MTT assay. The expression of VEGF was detected by enzyme-link immunoassay（ELISA）. Results The anti -tumor effect of monocytes/macrophages against Tca8113 cells in acid environment was lower than in normal environment（P<0.05）. VEGF excreted by mononuclear/macrophage was significantly higher in acid microenvironment than in normal microenvironment. Conclusion Due to acid microenvironment inside tumor, the anti-tumor effect of monocytes/macrophages against tumor cell was decreased, but the secretion of VEGF was gradually increased. However, the function of monocytes/macrophages on anti-tumor need more research.