磷酸化ErbB2(Tyr1248)对乳腺癌患者的诊断意义

目的 观察磷酸化ErbB2(Tyr1248)在乳腺癌发生、发展中的状况 ,探讨磷酸化ErbB2(Tyr1248)对乳腺癌患者的诊断意义。方法 用抗磷酸化ErbB2(Tyr1248)抗体检测乳腺癌细胞及癌旁“正常”乳腺上皮细胞的磷酸化ErbB2(Tyr1248)激活程度。用SPSS统计软件分析。结果 乳腺癌细胞与癌旁“正常”乳腺上皮细胞的磷酸化ErbB2(Tyr1248)激活程度差别有非常显著性意义 (U=535.000 ,P<0.001) ;不同组织学类型、有否淋巴结转移、患者年龄、病变部位之间磷酸化ErbB2(Tyr1248)激活程度无差别 (P>0.05) ;乳腺癌细胞的磷酸化ErbB2(Tyr1248)激活程度与ErbB2表达水平之间阳性分布有差别 (U=804.000,P<0.05) ,但磷酸化ErbB2(Tyr1248)的激活程度与ErbB2表达水平无等级相关性 (χ2=4.968,P>0.05)。结论 用抗磷酸化ErbB2(Tyr1248)抗体检测乳腺癌标本的磷酸化ErbB2(Tyr1248)激活程度对乳腺癌患者的诊断有参考价值。

The significance of phospho-HER2/Erb-B2 (Tyr1248) in the diagnosis of patients with breast cancer

Objective To detect the phosphorylation of Erb-B2(Tyr1248)in the breast cancer,and to explore the diagnostic significance of phospho_HER2/Erb_B2(Tyr1248)in patients with breast cancer.Methods The endogenous levels of Tyr1248(the phosphorylated Erb_B2)on the breast cancer cells and the epithelia cells of the mammary gland nearby the cancer were detected by anti_Tyr1248antibody.The data were analyzed by statistical software SPSS.Results The phosphorylation degree of Erb_B2(Tyr1248)in the breast cancer cells was higher than that in the epithelia cells of the mammary gland nearby the cancer(U=535.000,P<0.001).There was no significant difference in the levels of phosphorylated Erb_B2among different pathohistological types of the breast cancer and other clinical features in patients(P>0.05).There was some correlation between the activated degree of Erb_B2by phosphorylation and the levels of Erb-B2expression in breast cancers(U=804.000,P<0.05).Conclusion The activated degree of Erb-B2by phosphorylation may be of clinical significance in diagnosis of breast cancer.