肺鳞癌与人乳头瘤病毒相关性研究

目的:了解肺鳞癌组织中人乳头瘤病毒存在情况,并分析人乳头瘤病毒与肺癌发生部位、性别因素、角化程度、吸烟因素的关系.方法:采用地高辛标记的DNA探针对肺癌组织进行原位杂交检测,同时结合临床资料分析多种因素的相关性.结果:肺鳞癌组织,鳞状上皮生化组织、粘膜慢性炎组织HPV检出率分别为48.4%,27.4%,5.6%,各组比较差异显著(P＜0.01),中心型肺癌与周围型肺癌组织HPV检出率分别为50.6%和4.4%,两组比较差异显著(P＜0.01).男女两组HPV检出率分别为46.1%和54.3%,差异无显著性(P＞0.05).吸烟组HPV检出率为50.6%,非吸烟组为43.9%,差异不显著(P＞0.05).结论:肺癌组织中存在一定比例HPV感染,HPV感染与肺鳞癌关系密切,中心型肺癌与HPV感染密切相关,HPV感染与性别及吸烟因素未发现明显相关性.人乳头瘤病毒感染可能与肺癌发生发展有关.

The Studies on Human Papillomavirus Infection in Squamous Cancer of the Lung

Objective: To investigate the relationship between human papillomavirus (HPV) infection and lung cancer, observe the distribution of HPV-DNA in the tissue of lung cancer and to analyze the interrelationship among human papillomavirus, smoke, sex and the histological stage of lung cancer. Methods: Tissue of lung cancer was obtained by surgical resection or biopsy. The tissue specimens were detected by in situ hybridization techniques for human papillomavirus types 6B/11, 16, 18 and 31 using digoxingemin labelled DNA probe. The studies on the mechanisms involved in pulmonary carcinogenesis associated with HPV infection. Results: a, The positive sign was mainly located in the nuclei of the cells. The rate of 48.4% of squamous cell carcinomaoma, 27.4% of metaplasia and 5.6% of chronic inflammation tissue were positive for HPV-DNA by in situ hybridization (P<0.01); b, The positive rate of HPV in the central type group and peripheral typecontrol group was 50.6% and 4.4%, respectively (P<0.01). c. No significant difference in positive rate of HPV in smoke, sex and the histological stage of lung cancer were found in squamous cell carcinoma. Conclusion: The HPV-DNA is closely associated with squamous cell carcinoma of the lung, especially in the central type lung cancer. The correlationship between the HPV-DNA and smoke, the sex and the histological stage of lung cancer were not found.