局部深低温停循环对大鼠血清NSE的作用

目的通过研究深低温停循环和顺行性灌注大鼠脑复苏对大鼠神经元特异性烯醇化酶（NSE）的影响，探讨深低温停循环和顺行性灌注对脑的治疗及保护效果。方法制造大鼠损伤模型，将大鼠随机分为对照组（9只）；实验组（9只），用酶联免疫吸附法（ELISA）测定血浆中的NSE活性。结果实验组血清NSE活性在损伤后3、6、24h监测明显低于对照组（P〈0．01）。结论深低温停循环和顺行性灌注脑复苏技术通过降低NSE活性来增强脑细胞的自身保护作用可能是其抗神经细胞损伤的分子机制之一。

Effects of profound hypothermia with circulatory arrest on neuron specific enolase in rats

Objective To observe the effects of profound hypothermia with circulatory arrest on the change of neuron specific enolase （NSE） in serum in rats so as to evaluate the protective effect of the profound hypothermia with circulatory arrest on the brain. Methods A total of 18 rats were subjected to a cortical impact injury caused by a free-falling object and divided randomly into two groups： control group （9 rats） and experimental group （9 rats）. A series of samples were obtained 3, 6 and 24 h directly after trauma in two groups respectively. Enzyme-linked Immunosorbent assay （ELISA） was used to determine the serum of NSE. Results The serum level of NSE in rats was significantly decreased in experimental group compared with the control group, 3, 6 and 24 h after injury （P 〈0.01 ）. Conclusion Profound hypothermia with circulatory arrest can dramatically inhibit the release of NSE, enhance endurance of neurons to brain injury, and alleviate the severity of the damaged neurons.