烹调油油烟的潜在致癌性实验研究

为了研究烹调油油烟冷凝物对人体潜在的致癌危险性。以人胚肺二倍体细胞株建立人类细胞体外转化系统 ,将烹调油烟冷凝物作为受试物加入培养液中与细胞共培养 ,观察细胞转化情况 ,并对转化细胞的生物学性状检测和鉴定。结果表明试验剂量范围内的烹调油烟冷凝物均能诱导细胞转化 ,且剂量 反应关系明显 (r=0 9811) ,转化细胞表现出失去接触抑制性、饱和密度增大、血清需求降低、ConA凝集能力增强、染色体异倍化和锚着依赖性丧失等多种与肿瘤形成相关的表型改变。提示烹调油烟冷凝物具有诱导人胚肺细胞KMB 17恶性转化的作用 ,对人体具有潜在致癌危险性。

Experimental study on the potential carcinogenicity of cooking oil fume condensate

The potential carcinogencity of cooking oil fume condensate (COFC) to human was studied. Human embryo lung diploid fibroblast cell strain KMB-17 cell was applied to establish a human diploid cell transformation system in vitro. Different concentrations of COFC were added into the media and co-incubated with cells. The malignant degree of transformation was assessed by the biological characteristics of the cells. The concentrations of COFC within the dose range of the experiment could induce the malignant transformation of KMB-17 cell, and with a obvious dose-response relationship ( r =0 9811). Transformed cells have exhibited many characteristics associating with malignant transformation, such as loss of density and contact-dependent inhibition, growth at low serum concentration, agglutination by low concentration of Con A, alteration of karyotype from diploid to aneuploid, and lose of anchorage dependence. It suggested that the malignant transformation of human embryo lung diploid fibroblast cell strain KMB-17 cell could be induced by COFC, which might have potential carcinogenicity to human.