急性呼吸窘迫综合征血管内皮细胞功能改变的研究

为研究急性呼吸窘迫综合征血管内皮细胞功能改变，采用同种骨髓提取液（ＢＭＥ）复制兔急性呼吸窘迫综合征（ＡＲＤＳ）模型，连续多时相点检测血管内皮标记物；循环内皮细胞（ＣＥＣ），血浆颗粒膜蛋白－１４０（ＧＭＰ－１４０）和内皮素（ＥＴ－１）以及肺组织ＧＭＰ＿１４０含量，并行常规病理及电镜检查。发现致伤后０．５ｈ，ＣＥＣ，血浆ＧＭＰ－１４０，ＥＴ－１均显著升高，持续６ｈ仍显著高于伤前和对照，其中血浆ＧＭＰ－

A PRELIMINARY STUDY ON THE MARKERS OF VASCULAR ENDOTHELIAL CELLS IN ACUTE RESPIRATORY DISTRESS SYNDROME IN RABBITS

To explore the changes in the markers of vascular endothelial cells(VEC) in acute respiratory distress syndrome (ARDS) in rabbits. Thirty one rabbits were randomized into the control( n =10) and BME( n =21 Bone marrow extract, BME 0.35ml/kg 2ml/h) groups. They were sacrificed 6h after challenge. Circulating endothelial cell(CEC), granule membrane protein 140(GMP 140)and endothe lin 1(ET 1) were determined by means of immunoradiometric assay(IRMA), radioimmunoassay(RIA) and immunohistochemistry (IHC) respectively. The findings were as follows: CEC, GMP 140 and ET 1 were all increased in early phase(0.5h) and lasted for 6h. The dramatically elevated level of plasma GMP 140(3.25times) in early phase was negatively related to PaO 2, but positively to other corresponding parameters. The GMP 140 on the surface of PVEC displayed by IHC staining became weak. Conclusion: The method of injection of BME at slow rate can produce an acceptable model of ARDS. GMP 140 might be a satisfactory criterion for the early surveillance and for the evaluation of prognosis of ARDS in the clinical practice.