白念珠菌对氟康唑耐药的差异性膜蛋白质组学分析

目的 鉴定并分析白念珠菌氟康唑敏感株与耐药株的差异表达膜蛋白质。 方法 以同一亲本来源的白念珠菌氟康唑敏感株CA-3和耐药株CA-16为研究对象，以双向聚丙烯酰胺凝胶电泳（2D-PAGE）技术分析菌株之间膜蛋白图谱的差异，以基质辅助激光解吸电离飞行时间串联质谱（MALDI-TOF/TOF-MS）鉴定表达差异的蛋白质，通过白念珠菌蛋白质数据库进行检索分析。结果 白念珠菌氟康唑耐药株较敏感株有22种差异表达蛋白质点，其中有6种蛋白质上调，为Adh1p、Csp37p、Pgk1p、Pgk1p和2个未命名蛋白质（gi227305312、gi53954641）；16种蛋白质下调，分别为Aco1p、Aco1p、Hsp78p、Gut2p、Sdh12p、Ilv2p、Ndh51p、Ndh51p、Atp1p、Pda1p、Srb1p、Idh1p、Tdh1p、Cyt1p、Cox4p、Cox13p。结论 白念珠菌氟康唑耐药株较敏感株差异膜蛋白主要涉及能量代谢、线粒体功能等。 【关键词】 念珠菌，白色； 氟康唑； 抗药性，真菌； 膜蛋白质类； 蛋白质组学

Proteomic analysis of plasma membrane from fluconazole-resistant Candida albicans

ZHANG Xiao-li, GAO Lai-qiang, WU Xi-fu, ZHANG Hong *, ZHANG Ge-hua, YIN Xing-feng. *First Affiliated Hospital, Institute of Mycology, Jinan University, Guangzhou 510632, China Corresponding author： ZHANG Hong, Email: tzhang@jnu.edu.cn 【Abstract】 Objective To identify and analyze plasma membrane proteins differentially expressed between fluconazole-sensitive and -resistant C.albicans strains. Methods Two C.albicans strains from a same parent, including the fluconazole-sensitive C.albicans strain CA-3 and fluconazole-resistant C.albicans strain CA-16, served as the subject of this study. Plasma membrane proteins were isolated from both of the C.albicans strains, and subjected to two-dimensional polyacrylamide gel electrophoresis analysis for the screening of differentially expressed proteins, which were then identified by using matrix assisted laser desorption/ionization time-of-flight mass spectrometry. The resultant data were searched against a protein database for C.albicans. Results Twenty-two proteins were identified to be differentially expressed between the fluconazole-resistant and -sensitive C.albicans strain. Of them, 6 proteins（Adh1p, Csp37p, Pgk1p, Pgk1p and 2 unnamed proteins, i.e., gi227305312 and gi53954641） were highly expressed, while 16 proteins （Aco1p, Aco1p, Hsp78p, Gut2p, Sdh12p, Ilv2p, Ndh51p, Ndh51p, Atp1p, Pda1p, Srb1p, Idh1p, Tdh1p, Cyt1p, Cox4p, Cox13p） were lowly expressed in the fluconazole-resistant C.albicans strain compared with the fluconazole-sensitive strain. Conclusion The plasma membrane proteins differentially expressed between fluconazole-sensitive and -resistant C.albicans strain are mainly implicated in energy metabolism and mitochondrial function. 【Key words】 Candida albicans; Fluconazole; Drug resistance, fungal; Membrane proteins; Proteomics