饱和氢氯化钠溶液对小鼠变应性接触性皮炎的影响

目的 探讨饱和氢氯化钠溶液对变应性接触性皮炎（ACD）的治疗作用及相关机制。方法 将40只小鼠随机分为四组：对照组、对照 + 饱和氢氯化钠溶液组、ACD组和ACD + 饱和氢氯化钠溶液组。将二硝基氟苯搽在小鼠外耳建立二硝基氟苯致小鼠变应性接触性皮炎模型，腹腔注射饱和氢氯化钠溶液5 ml/kg进行治疗。评估皮损，计算耳厚度差、耳肿胀度，酶联免疫吸附试验检测皮损组织肿瘤坏死因子α（TNF-α）、白介素（IL）-6、IL-17和干扰素γ（IFN-γ）水平，并计数皮损中炎性细胞数量。采用SPSS 18.0统计软件进行数据分析，各组间比较采用单因素方差分析，两两比较采用LSD分析法。 结果 与对照组比较，ACD组皮损评分（7.33 ± 1.53）、耳厚度差［（0.73 ± 0.15） mm］和耳肿胀度［（18.67 ± 3.05） mg］增加，左耳皮损组织TNF-α［（1475.52 ± 233.81） ng/g］、IL-6［（184.65 ± 78.39） ng/g］、IL-17［（628.56 ± 201.44） ng/g］和IFN-γ［（197.72 ± 37.81） ng/g］水平和炎性细胞数量［（752.00 ± 166.06）个/mm2］亦增加（均P < 0.05）；与ACD组比较，ACD + 饱和氢氯化钠溶液组皮损评分（3.33 ± 0.58）、耳厚度差［（0.46 ± 0.11） mm］、耳肿胀度［（11.00 ± 2.64） mg］、左耳皮损组织TNF-α［（817.72 ± 101.13） ng/g］、IL-6［（95.86 ± 36.65） ng/g］、IL-17［（373.38 ± 126.74） ng/g］和IFN-γ［（63.31 ± 17.38） ng/g］水平和炎性细胞数量［（384.00 ± 97.35）个/mm2］均降低（均P < 0.05）。结论 饱和氢氯化钠可以减轻ACD中炎症因子的释放。

Effect of hydrogen-rich saline on allergic contact dermatitis in mice

MENG Chen-yang *, CHEN Hong-guang, MENG Xiao-yin, XING Wei-bin, FU Guo-jun. *Hebei Cangzhou Medical College, Cangzhou 061001, Hebei, ChinaCorresponding author: FU Guo-jun, Email: Fgj8899@163.com 【Abstract】 Objective To evaluate the therapeutic effect of hydrogen-rich saline on allergic contact dermatitis （ACD） in mice and to explore its underlying mechanisms. Methods Forty mice were equally divided into 4 groups: control group, control treatment group, ACD group and ACD treatment group. ACD was induced by repetitive topical application of dinitrofluorobene（DNFB） to the left ear of mice on day 1, 2 and 5. Hydrogen-rich saline was intraperitoneally given to the mice in the ACD treatment group at a dose of 5 ml/kg per day from day 1 to 5. On day 6, the mice were sacrificed, ear tissue was removed from them and subjected to measurement of thickness and weight, detection of tumor necrosis factor （TNF）-α, interleukin （IL）-6, IL-17 and interferon （IFN）-γ expression by enzyme linked immunosorbent assay, as well as numeration of inflammatory cells in lesions after hematoxylin-eosin （HE） staining. Data were processed by SPSS 18.0 software, and statistical analysis was carried out by one-way analysis of variance and least significant difference （LSD） procedure. Results Compared with the control group, the ACD group showed a significant increase in lesion score （7.33 ± 1.53 vs. 0, P < 0.05）, differences in the thickness （（0.73 ± 0.15） mm vs. （0.13 ± 0.05） mm, P < 0.05） and swelling degree （expressed as tissue weight: （18.67 ± 3.05） mg vs. （3.33 ± 1.52） mg, P < 0.05） between the left and right ear, expressions of TNF-α（（1475.52 ± 233.81） pg/mg vs. （239.01 ± 52.39） pg/mg, P < 0.05）, IL-6（（184.65 ± 78.39） pg/mg vs. （42.28 ± 17.64） pg/mg, P < 0.05）, IL-17 （（628.56 ± 201.44） pg/mg vs. （127.58 ± 50.28） pg/mg, P < 0.05） and IFN-γ（（197.72 ± 37.81） pg/mg vs. （24.57 ± 8.31） pg/mg, P < 0.05）, and the number of inflammatory cells per square millimetre in the left ear tissue （752.00 ± 166.06 vs. 127.33 ± 77.18 , P < 0.05） . However, hydrogen-rich saline treatment induced a statistical decrease in all of these parameters in the ACD treatment group compared with the ACD group, including lesion score （3.33 ± 0.58, P < 0.05）, difference in thickness （（0.46 ± 0.11） mm, P < 0.05） and swelling degree （（11.00 ± 2.64） mg, P < 0.05）, expressions of TNF-α （（817.72 ± 101.13） pg/mg, P < 0.05），IL-6 （（95.86 ± 36.65） pg/mg, P < 0.05），IL-17 （（373.38 ± 126.74） pg/mg, P < 0.05）, IFN-γ （（63.31 ± 17.38） pg/mg, P < 0.05） and the number of inflammatory cells per square millimetre（384.00 ± 97.35, P < 0.05）. Conclusion Hydrogen may inhibit the release of inflammatory factors in ACD.