HPLC-ELSD法测定乌灵菌粉中甘露醇的含量

目的建立HPLC-ELSD法测定鸟灵菌粉中甘露醇的含量。方法采用氨基柱（Ultimate XB-NH2，4．6mm×250mm，5μm），柱温：25℃，进样量：20μL，以乙腈-水（84：16）为流动相洗脱，流速：1．0mL·min^-1；ELSD漂移管温度：95℃，氮气压力：30psi。结果甘露醇在332-1992μg·mL^-1浓度范围内线性关系良好（r=0．9992，n=6）；方法回收率为98．86%，RSD为1．01%。结论该方法准确度高、专属性强、重复性好，可用于乌灵菌粉的质量控制。

Content Determination of Mannitol in Wuling Powder by HPLC-ELSD

Objective To establish an HPLC-ELSD method to determine the content of mannitol in Wuling Powder. Methods The determination was performed on an amino chromatographic column （Ultimate XB-NH2, 4.6 min×250 mm, 5μm） with a mobile phase of acetonitrile-water （84 ： 16）. The flow rate was 1.0 mL · min^-1, the temperature of the ELSD drift tube was set at 95℃, and the nitrogen pressure was 30 psi. The injection dosage was 20 μL. Results A good linear correlation （r = 0. 9992） was observed when the content of mannitol was in the range of 332-1992 μg ·mL^-1. The recovery of the method was 98.86%, and the relative standard deviation （RSD） was 1.01% （n=6）. Conclusion This method is reliable, accurate, with high sensitivity and repeatability. It can be used for quality control of Wuling Powder.