MiR-150在大鼠垂体瘤细胞中的表达及其对细胞增殖的影响

目的 探讨微小核糖核酸-150(miR-150)在大鼠垂体瘤细胞中的表达及其对垂体细胞增殖的影响.方法 选用5周龄Fischer344雌性大鼠20只,按实验要求分为对照组(n=10)与观察组(垂体瘤组,n=10).观察组大鼠皮下埋置10 mg雌激素缓释泵诱导垂体瘤模型,对照组小鼠皮下埋置生理盐水缓释泵作为对照.造模成功后,观察组及对照组分别取垂体瘤组织及正常垂体组织,采用实时荧光定量PCR(qPCR)检测miR-150的表达.培养正常的大鼠垂体细胞,用miR-150 mimics和miR-150 mimics control分别转染正常的垂体细胞,并观察其对细胞增殖功能的影响.结果 结果显示观察组组织中miR-150的表达量为(0.39±0.10),明显低于对照组的(1.47±0.37),差异有统计学意义(P<0.05);转染miR-150 mimics的正常垂体细胞增殖(1.16±0.11),相对于转染miR-150 mimics control的(1.82±0.13)显著减少,差异有统计学意义(P<0.05).结论 miR-150在垂体瘤中表达下调,其可能调控垂体细胞的增殖从而抑制垂体瘤的发生.

Expression of microRNA-150 and its effect on cell proliferation of microRNA-150 in pituitary tumor cells of rats

Objective To investigate the expression of microRNA-150 (miR-150) and its effect on cell prolif-eration in pituitary tumor cells of rats. Methods Twenty Fischer344 female rats of five weeks old were selected. They were divided into control group (n=10) and observation group (pituitary adenoma group, n=10) according to experiment requirements. The observation group was treated by embedding estrogen slow-release pumps subcutaneously to induce model of pituitary adenoma, and the control group were treated with saline sustained-release pump subcutaneously as con-trol. After successful modeling, pituitary adenoma and normal pituitary tissue were respectively extracted in the observa-tion group and the control group. The expressions of miR-150 was detected by real-time quantitative PCR (qPCR). Hu-man pituitary adenoma cells were cultured in vitro. MiR-150 mimics and miR-150 mimics control were respectively transfected into normal pituitary cells, and their effect on cell proliferation function was observed. Results MiRNA-150 expression in observation group was (0.39 ± 0.10), which was significantly lower than (1.47 ± 0.37) in control group (P<0.05). The cell proliferation of normal pituitary cells transfected with miRNA-150 mimics was (1.16 ± 0.11), which was significantly lower than (1.82±0.13) of those transfection with miR-150 mimics control (P<0.05). Conclusion Expres-sion of miRNA-150 is decreased in pituitary adenoma, which may regulate the proliferation of pituitary cells, thereby in-hibiting the occurrence of pituitary adenomas.