lncRNA HOTAIR在宫颈癌组织及HeLa细胞中的表达及其生物学功能研究

目的 探讨长链非编码RNA HOTAIR在宫颈癌组织中的表达及其生物学功能.方法 选取简阳市人民医院2014年8月至2016年7月收治并手术治疗的宫颈癌患者47例,采用实时荧光定量PCR检测患者癌组织及癌旁正常宫颈组织中HOTAIR RNA的相对表达量;以人宫颈癌Hela细胞为研究材料,应用小RNA干扰技术下调宫颈癌Hela癌细胞HOTAIR RNA表达,分别应用CCK-8实验、Wound Healing实验检测小干扰RNA下调HOTAIR表达前后Hela细胞增殖和迁移能力变化情况.结果 HOTAIR在宫颈癌患者癌组织与癌旁正常宫颈组织中的相对表达量分别为(6.89±2.12)与(4.02±1.89),癌组织显著高于癌旁组织,差异有统计学意义(P<0.05);与对照组细胞相比,外源性小干扰RNA可显著敲低Hela细胞中HOTAIR的表达并影响其增殖和迁移(P<0.05).结论 宫颈癌患者癌组织中HOTAIR表达水平高于癌旁正常宫颈上皮;HOTAIR可能参与了宫颈癌细胞增殖和迁移等生物学功能.

Long non-coding RNA HOTAIR expression in cervical cancer tissue and HeLa cells and its biology function

Objective To discuss the long non-coding RNA HOTAIR expression in cervical cancer tissue, cor-responding normal tissue and its biology function. Methods Forty-seven cervical cancer patients who received opera-tion for cervical cancer were included in this study from Aug. 2014 to Jul. 2016. HOTAIR relative expression was tested by real-time PCR in the cancer tissue and corresponding normal tissue. HOTAIR was knock down in HeLa cells by small interfering RNA (shRNA). CCK-8 assay was used to detect the cell proliferation ability, and the wound healing as-say was used to detect the migration ability after inhibiting HOTAIR expression in HeLa cells. Results The relative HOTAIR expression in cervical cancer and normal tissue were (6.89±2.12) and (4.02±1.89), with statistically significant difference (P<0.05). shRNA_HOTAIR could successfully decrease HOTAIR expression in HeLa cells, and the prolifera-tion and migration ability were inhibited by knocking down HOTAIR in HeLa cells compared to shRNA_control cell (P<0.05). Conclusion HOTAIR is highly expressed in cervical cancer tissue and plays important roles in the proliferation and migration of HeLa cell.