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An ELISA to detect antibody specific for surface antigens of parasitic nematodes
Authors:L L Schroeder
Affiliation:Department of Zoology, University of Massachusetts, Amherst, MA 01003-0027, U.S.A.
Abstract:An enzyme immunoassay method is described for the detection of specific immunoglobulins bound to the surface of intact adult Nippostrongylus brasiliensis. Binding of antibody is complete in 1 h and the entire procedure can be completed in less than 4 h. This method has been used to detect the quantity of specific IgG antibodies in sera of rats infected with N. brasiliensis.
Keywords:ELISA  surface antigens  nematodes  Nippostrongylus brasiliensis
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