An ELISA to detect antibody specific for surface antigens of parasitic nematodes |
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Authors: | L L Schroeder |
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Affiliation: | Department of Zoology, University of Massachusetts, Amherst, MA 01003-0027, U.S.A. |
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Abstract: | An enzyme immunoassay method is described for the detection of specific immunoglobulins bound to the surface of intact adult Nippostrongylus brasiliensis. Binding of antibody is complete in 1 h and the entire procedure can be completed in less than 4 h. This method has been used to detect the quantity of specific IgG antibodies in sera of rats infected with N. brasiliensis. |
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Keywords: | ELISA surface antigens nematodes Nippostrongylus brasiliensis |
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