不同临床期Graves''病患者外周血分泌TRAb前体细胞频率的差异

目的：探讨分泌TRAb的前体细胞频率与Graves‘病临床分期的关系。方法：利用EB病毒体外激活外周血淋巴细胞，测定不同临床期GD患者外周血中潜在的TRAb分泌细胞的频率差异。结果：血清TRAb较高的初发和复发期患者的分泌TRAb的转化B细胞阳性孔占免疫球蛋白阳性孔的百分率远高于正常对照，2例初发病人的阳性率分别为34．6％和29．1％，1例复发病人阳性率为30％，正常对照中也可以检出较低频率的TRAb前体细胞，2例血清TRAb阴性的缓解期病人的外周血中，其中1例分泌TRAb前体细胞频率（5．3％）与正常无差异，另1例TRAb前体细胞频率（10．7％）高于正常对照。EBV转化的TRAb前体细胞以分泌IgM型抗体为主。结论：不同临床期的Graves‘病患者的外周血中分泌TRAb的前体细胞的频率不同。

Different frequencies of B lymphocytes committed to produce anti-thyrotropin receptor antibodies in patients at different clinical stages of Graves'''' disease

Objective To investigate the relationship between the TRAb producing cell precursors and different clinical stages of Graves' disease. Methods Peripheral lymphocytes were infected with Epstein Barr virus, a kind of lymphocyte precursor stimulator, and were stimulated to produce TRAb. Results Of the total wells containing Ig producing B cell precursors, there were 34.6% and 29.1% from 2 untreated patients, 30% from 1 relapsing patient and only 1.3% and 3.8% from 2 healthy controls that secreted TRAb, respectively. As for the wells containing B cell precursors from 2 remission patients with undetectable TRAb in circulation, the percentage (10.7%) of TRAb containing wells from one was higher than that from the other (5.3%). And the latter had similar percentage of TRAb positive wells with the controls. The isotype of TRAb in supernatants of our EBV transformed B lymphocytes was predominantly IgM. Conclusion The frequencies of TRAb specific precursors were different at three distinct clinical stages of the disease. [