不同方法检测外周血嗜酸性粒细胞计数准确性比较

目的比较五种方法检测外周血嗜酸性粒细胞计数的准确性。方法对100例健康体检正常者分别采用显微镜直接计数、三种不同产地的五分类血细胞自动分析仪、流式细胞仪等五种方法进行外周血嗜酸性粒细胞检测。结果100例血标本检测结果：显微镜计数法为（0．249±0．112）×10^9／L；BC-5500、XE-2100和AC-T5diff血细胞分析仪所测结果分别为（0．253±0．105）×10^9／L、（0．252±0．103）×10^9／L和（0．256±0．104）×10^9／L；流式细胞仪所测结果为（0．259±0．101）×10^9／L，五种方法结果差异无统计学意义（F=1．225，P〉0．10）。结论五种方法检测外周血嗜酸性粒细胞计数均较准确，五分类血细胞自动分析仪和流式细胞仪操作简便，重复性好，但仪器设备及试剂较昂贵；显微镜直接计数方法操作较烦琐，重复性稍差。

Comparisom of different methods of determination of eosinophil cells

Objective To comparisom five methods of detection of eosinophil cells,in order to analyze the precision of the five methods.Methods The eosinophil cells of 100 patients were tested by using microscope、BC T5difft and flow cytometry are(0.249 ±0.112) × 109/L,(0.253 ±0.105) × 109/L,(0.252 ±0.103) × 109/L,(0.256 ±0.104) × 109/L and(0.259 ±0.101) × 109/L.There are no statistical differences(F = 1.225,P＞0.10)among the five methods.Conclusion The results of the five methods are all accurate;the automatic analyzer method and the flow cytometry method are simple and repeatable,but the equipment and reagents are more expensive;the microscope direct counting method is more complicated and less repeatable.