甲基毒死蜱的代谢转化机制研究

目的探讨甲基毒死蜱在大鼠体内的代谢转化机制。方法应用体外代谢系统对甲基毒死蜱的代谢机制进行研究,蛋白含量按Lowry法测定,甲基毒死蜱含量采用气相色谱法测定,甲基毒死蜱代谢产物的分离采用高效液相色谱法。结果甲基毒死蜱主要是在机体的肝脏被代谢转化,肝细胞的微粒体和可溶性胞浆是主要的代谢转化场所。对甲基毒死蜱的代谢产物进行分离发现,在肝微粒体中存在明显的氧化代谢作用,微粒体和可溶性胞浆对甲基毒死蜱的代谢存在着不完全相同的生物转化系统。结论甲基毒死蜱的主要代谢场所是肝细胞的微粒体和可溶性胞浆,氧化代谢是其主要的代谢方式。

Study on Metabolic Mechanisms of Chlorpyrifos - methyl

ObjectiveTo investigate the metabolic mechanisms of chlorpyrifos-methyl in vitro of rat.MethodsMetabolic system was used to study the metabolic mechanism of chlorpyrifos-methyl.The protein concentration of tissue homogenates and cell fractions were determined according to the method of Lowry.The content of chlorpyrifos-methyl was determined by gas chromatography.In vitro metabolic test,chlorpyrifos-methyl and its metablites were assayed by high-performance liquid chromatography.ResultsChlorpyrifos-methyl was mainly metabolized in the liver of rats.In liver cells,the main place of metabolism of chlorpyrifos-methyl was hepatic microsomes and cytoplasm.The obvious oxidation of chlorpyrifos-methyl was catalyzed by microsomal enzymes.The different systems of biotransformation for chlorpyrifos-methyl existed in hepatic microsomes and cytoplasm.ConclusionThe most important site of metabolism of chlorpyrifos-methyl was hepatic microsomes and cytoplasm.The metabolic mode of chlorpyrifos-methyl was mostly oxidation reaction.