沙生蜡菊花中黄酮类成分的分离与鉴定

目的研究沙生蜡菊(Helichrysum arenarium(L.)Moench)花的化学成分。方法采用硅胶柱色谱?ODS柱色谱和HPLC柱色谱分离纯化,依据理化性质、波谱数据分析进行结构鉴定。结果从沙生蜡菊花的甲醇提取物中分离得到8个黄酮类化合物,分别鉴定为山奈酚3-O-β-D-吡喃葡萄糖苷(kaempferol 3-O-β-D-glucopyranoside,1)、木犀草素3′-O-β-D-吡喃葡萄糖苷(luteolin3′-O-β-D-glucopyranoside,2)、木犀草素7-O-β-D-吡喃葡萄糖苷(luteolin7-O-β-D-glucopyranoside,3)、木犀草素6-羟基-7-O-β-D-吡喃葡萄糖苷(luteolin6-hydroxy-7-O-β-D-glucopyranoside,4)、木犀草素3′-甲氧基-6-羟基-7-O-β-D-吡喃葡萄糖苷(luteolin3′-methoxyl-6-hydroxy-7-O-β-D-glucopyranoside,5)、黄芩素7-O-β-D-吡喃葡萄糖苷(scutellarein7-O-β-D-glucopyranoside,6)、山柰酚3-O-β-D-龙胆二糖苷(kaempferol3-gentiobioside,7)、山柰酚3-O-(3-β-D-吡喃葡萄糖基)-β-D-吡喃葡萄糖苷(kaempferol3-O-(3-β-D-glucopyranosyl)-β-D-glucopyranoside,8)。结论化合物2、4~8为首次从蜡菊属植物中分离得到。

Isolation and identification of chemical constituents of flavones from Flos Helichrysi Arenarii

Objective To study the chemical constituents of Flos Helichrysi Arenarii. Method The chemical constituents were isolated by various isolation methods (silica gel, ODS and HPLC column chromatography) and their structures were elucidated by the analysis of spectral data and chemical properties. Result Eight flavones compounds were isolated from the MeOH extract of Flos Helichrysi Arenarii. The chemical structures were elucidated as kaempferol 3-O-β-D-glucopyranoside (1), luteolin 3’-O-β-D-glucopyranoside (2), luteolin 7-O-β-D-glucopyranoside (3), luteolin 6-hydroxy-7-O-β-D- glucopyranoside (4), luteolin 3-methoxyl-6-hydroxy-7-O-β-D-glucopyranoside (5), scutellarein 7-O-β-D-glucopyranoside (6), kaempferol 3-gentiobioside (7), kaempferol 3-O-(3-β-D- glucopyranosyl)-β-D-glucopyranoside (8). Conclusion Compound 2, 4-8 are obtained from Helichrysum Mill. for the first time.