核仁组织区嗜银蛋白与脑胶质瘤恶性程度关系的研究

目的 研究脑胶质瘤细胞中AgNOR染色与Ki-67阳性表达间的关系以及与病人预后的关系。方法 45例脑胶质瘤标本行AgNOR染色。13例新鲜脑胶质瘤标本同时行Ki-67免疫组化染色。30例获随访病例作AgNOR计数的生存统计。结果 AgNOR计数与组织病理发级正相关并与Ki-67呈线性正相关。AgNOR计数＜1．80病例组预后明显好于AgNOR计数≥1．80病例组。结论 AgNOR计数是一项反映了脑胶质瘤良恶性程度的定量指标，可作为肿瘤细胞动力学参数的间接指标，并与病人预后相关。

Silver colloid staining technique of Ag-NOR'S for analysis of glioma malignancy

Objective To identify the number of Ag NOR'S per cell correlating with Ki 67 LI and patients prognosis in different histopathological grade human gliomas.Methods A silver colloid staining technique was applied to 45 human gliomas. In 13 cases, studies were performed in the number of Ag NOR'S and the PF using immunohistological study with the monoclonal antibody Ki 67. In 30 patients, the correlation between the number of Ag NOR'S and the prognosis was estimated.Results Significant differences among these were recognized, and the mean number of Ag NOR'S paralleled the degree of histopathological malignancy. A linear relationship was demonstrated between these two factors. The results demonstrated that the group containing patients with less than 1.8 Ag NOR'S per cell had a better progonsis than the group with 1.8 Ag NOR'S or more.Conclusion Silver colloid staining for Ag NOR'S is a simple, rapid and reproducible method for estimating the proliferative potential of human gliomas without requiring a complicated technique.