不同剂量卡托普利对病毒性心肌炎小鼠心肌线粒体的保护效应

目的研究不同剂量卡托普利对病毒性心肌炎小鼠心肌线粒体结构和ATP酶活性变化的影响。方法雄性Balb/c小鼠随机分为柯萨奇病毒B3（CVB3）感染组、CVB3感染加不同剂量卡托普利治疗组（10、30、100mg/kg,2次/d）和对照组。d14电镜观察线粒体超微结构,生化方法测定线粒体Na＋-K＋-ATP酶、Ca2＋-ATP酶活性。结果各剂量卡托普利治疗组线粒体膜Na＋-K＋-ATP酶、Ca2＋-ATP酶活性均较感染组高,但小剂量组差异无显著性（P〉0.05）,且线粒体溶解破坏,失去完整性;中、大剂量组差异具有显著性（P〈0.01）,且线粒体结构改变与同病变期感染组比较明显较轻。结论小剂量卡托普利治疗无明显心肌线粒体保护效应,中、大剂量卡托普利心肌线粒体保护效应明显。

Mitochondria Injury due to Viral Myocarditis Improved by Different Doses of Captopril in Mice

Objective To further understand the cellular events of myocarditis,we have examined the myocardial mitochondria structure and activity of ATPase in mice with myocarditis,and observe the interventional effect of different doses of carptopril.Methods Sixty male Balb/c mice were randomly divided into 5 groups:infected coxsackie B3 virus(CVB3)group,infected CVB3 and treated with carptopril(in a dose of 10,30,or 100 mg/kg,twice a day)groups and control group.Captopril was administered after infection.The activity of Na -K -adenosine triphosphatase(Na -K -ATPase)and Ca2 -adenosine triphosphatase(Ca2 -ATPase)of myocardial mitochondrial as well as the morphological of myocardial mitochondrial were investigated on day 14.Results The activity of mitochondrial Na -K -ATPase and Ca2 -ATPase were significantly higher(Pa<0.05)and mitochondria injury was less in mice receiving 30 or 100 mg/kg,twice a day,but not in mice receiving 10 mg/kg(P>0.05).Conclusions The favorable effects of captopril exerts on myocyte mitochondria were shown in a dose-dependent manner.Thirty and 100 mg/kg,twice a day captopril protecs the membrane integrity and thus plays a role at the recovery of depressed mitochondrial Na -K -ATPase and Ca2 -ATPase activity and also in myocytes injury.