微囊化异种嗅球组织移植联合药物干预修复受损脊髓的研究

目的:研究微囊化异种嗅球组织移植联合β-七叶皂甙钠对脊髓继发性损伤的治疗保护作用及机制。方法:健康SD大鼠180只,随机分为损伤对照组、微囊化兔嗅球组织移植组、微囊化兔嗅球组织移植联合β-七叶皂甙钠治疗组,损伤组又设1、3、7、14、28d5个时相点,紫外分光光度计检测脊髓伊文氏蓝含量;免疫组织化学观察血管内皮生长因子(VEGF)的表达;神经功能评分法(BBB法)评价大鼠术后运动功能的恢复情况。结果:脊髓损伤后脊髓伊文氏蓝含量增加、VEGF表达下调,大鼠的运动功能缺失,经微囊化异种嗅球组织移植联合β-七叶皂甙钠治疗后血脊髓屏障的通透性有所改善,VEGF表达上调,大鼠的运动功能有所恢复。结论:微囊化异种嗅球组织移植对脊髓继发性损伤有较好的疗效,联合应用嗅球组织细胞和β-七叶皂甙钠在脊髓损伤修复治疗中具有协同作用。

Combining exnotransplantation of microencapsulated olfactory bulb and drug intervention to repair the injured spinal cord

Objective:To explore the protective effects and mechanism of exnotransplantation of microencapsulated olfactory bulb combined with β-aescinate sodium in rats at the early stages of spinal cord injury.Methods:Healthy 180 SD rats were divided into three groups as following:injury control group,microencapsulated transplantation of rabbit olfactory bulb cells group,microencapsulated transplantation of rabbit olfactory bulb cells combined with β-aescinate sodium group,the later two groups were quantified at 1,3,7,14,and 28 d after spinal cord injury.Evans Blue content in the spinal cord was examined with the ultraviolet spectrophotometer;the change of vascular endothelial growth factor(VEGF) expression after spinal cord injury was detected by immunohistochemical method.BBB score was designed to assess its behavioral ability.Results:Evans Blue content was increased and expression of VEGF were significantly decreased,loss of motor function in rats after spinal cord injury was observed.Permeability of blood-spinal barrier was improved,VEGF was obviously upregulated,and motor function was recovered in rats after they were treated with microencapsulated transplantation of rabbit olfactory bulb cells combined with β-aescinate sodium.Conclusion:Exnotransplantation of microencapsulated strategy of olfactory ensheating cells was effective in the repair of spinal cord injury in certain extent.There may be a synergistic effect between olfactory ensheating cells and β-aescinate sodium.