当归补血、活血作用的谱效关系研究

用乙酰苯肼(APH)复制血虚小鼠模型,于实验第1,4,7 d皮下注射APH,剂量为200,100,100 mg·kg^-1(0.01 mL·g^-1)。于造模第10 天开始灌胃给予当归提取物 (生药剂量为2.4 g·kg^-1),连续10 d。观察当归不同提取部位对血虚小鼠RBC、Hb、PLT、胸腺、脾脏及体重变化等指标的影响。用灰色关联统计方法将HPLC指纹图谱各共有峰的峰面积和补血、活血药效数据相关联,研究谱效相关性。结果显示,水提醇沉上清(DSC)部位补血、活血效果较好。根据关联度大小确定了DSC中各成分对补血、活血作用贡献程度。其中,阿魏酸的贡献最大,但不应忽略其他成分的贡献。该文研究有效组分的HPLC指纹图谱及谱效关系,确定了DSC部分补血、活血的物质基础,为表征其谱效相关性提供有效途径。

Investigation on chromatogram-pharmacodynamics relationship of Angelica sinensis on effect of replenishing blood

Blood deficiency model of mice was copied by subcutaneous injection with 200, 100 and 100 mg·kg^-1(0.01 mL·g^-1)acetyl phenylhydrazine (APH) at the frist, fourth, and seventh days. Mice in each group were perfused with different extracted parts of Angelica sinensis (drug dosage was 2.4 g·kg^-1) at the tenth day, once a day for 10 days.Then compare the influence of different extracted parts of Angelica sinensis to RBC, Hb, PLT and thymus, spleen and weight changes of blood deficiency mice. The peak areas of each common peak from HPLC fingerprint were associated with the date of replenishing blood pharmacodynamics efficacy by using gray relation statistic, which was used to research the chromatogram-pharmacodynamics relationship.The results showed that the part of DSC has the better effect in replenishing blood. The contribution degree of the DSC to replenishing blood of each component were determined by correlation size, and ferulic acid made the largest contribution, but contribution of other components should not be ignored. In this paper, we research the relationship of the HPLC fingerprint and spectrum activity relationship, determine the material basis of the DSC for replenishing blood, and provide effective way to represent the spectral correlation effect.