| 利用UPLC-Q-TOF-MSE快速分析四物汤中的化学成分 |
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| 引用本文： | 王镇方,赵阳,庞旭,余河水,康利平,高月,马百平. 利用UPLC-Q-TOF-MSE快速分析四物汤中的化学成分[J]. 中国中药杂志, 2013, 38(21): 3702-3708 |
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| 作者姓名： | 王镇方 赵阳 庞旭 余河水 康利平 高月 马百平 |
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| 作者单位： | 军事医学科学院 放射与辐射医学研究所, 北京 100850;天津中医药大学, 天津 300193;军事医学科学院 放射与辐射医学研究所, 北京 100850;军事医学科学院 放射与辐射医学研究所, 北京 100850;军事医学科学院 放射与辐射医学研究所, 北京 100850;天津中医药大学, 天津 300193;军事医学科学院 放射与辐射医学研究所, 北京 100850;军事医学科学院 放射与辐射医学研究所, 北京 100850;军事医学科学院 放射与辐射医学研究所, 北京 100850 |
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| 基金项目： | 国家科技支撑计划项目(2008BAI51B02);国家自然科学基金重点项目(81130067) |
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| 摘 要： | 该研究利用UPLC-Q-TOF-MSE技术分析了四物汤的化学成分。通过正、负离子模式的检测,根据质谱数据以及相关文献,快速表征了43个色谱峰,鉴定了其中的25个化合物,主要包括来自白芍的8个单萜糖苷类化合物和来自川芎、当归的13个苯酞类化合物。同时,通过MarkerLynx软件进行主成分分析和偏最小二乘法分析,比较了四物汤水提液和60%乙醇提取液中的化学成分差异,为探讨四物汤补血作用的物质基础提供了依据。
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| 关 键 词： | 四物汤 UPLC-Q-TOF-MSE 化学成分 MarkerLynx 提取方法 |
| 收稿时间： | 2013-07-18 |
Analysis and identification of chemical constituents in Siwu decoction by UPLC-Q-TOF-MSE |
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| WANG Zhen-fang,ZHAO Yang,PANG Xu,YU He-shui,KANG Li-ping,GAO Yue and MA Bai-ping. Analysis and identification of chemical constituents in Siwu decoction by UPLC-Q-TOF-MSE[J]. China Journal of Chinese Materia Medica, 2013, 38(21): 3702-3708 |
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| Authors: | WANG Zhen-fang ZHAO Yang PANG Xu YU He-shui KANG Li-ping GAO Yue MA Bai-ping |
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| Affiliation: | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China;Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China;Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China;Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China;Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China;Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China;Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China |
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| Abstract: | This research analyzed the chemical constituents of Siwu decoction by UPLC-Q-TOF-MSE. Base on the data of mass and related-literatures, 43 peaks were profiled and 25 compounds, which contain 8 monoterpene glycosides from Paeonia lactiflora and 13 phthalides from Rhizoma chuanxiong and Radix angelica sinensis mainly, were identified in both positive and negative mode respectively. Meanwhile, chemical constituents of water extract and 60% ethanol extract of Siwu decoction were compared by the principal constituent analysis with MarkerLynx software, which provides the basis for the active ingredients of Siwu decoction. |
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| Keywords: | Siwu decoction UPLC-Q-TOF-MSE chemical constituents MarkerLynx method of extraction |
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