Transformation of Penicillium chrysogenum using the Aspergillus nidulans amdS gene as a dominant selective marker |
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| Authors: | Raj K. Beri Geoffrey Turner |
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| Affiliation: | (1) Department of Microbiology, University of Bristol, BS8 1TD Bristol, UK;(2) Present address: Department of Genetics, School of Biological Sciences, University of Leicester, LE1 7RH Leicester, UK |
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| Abstract: | Summary The Aspergillus nidulans acetamidase gene (amdS) has been used to transform Penicillium chrysogenum at low frequency. Several transformants were tested and shown to be mitotically stable. Southern blot analysis indicated that transforming DNA had integrated into the chromosomal DNA, possibly at multiple sites. |
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| Keywords: | Penicillium chrysogenum Transformation Acetamidase gene |
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