IgM-class antibodies to TT virus (TTV) in patients with acute TTV infection |
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| Affiliation: | 1. Graduate Program in Functional and Molecular Biology, Institute of Biology, University of Campinas, Campinas, SP, Brazil;2. Brazilian Biosciences National Laboratory (LNBio), Brazilian Center for Research in Energy and Materials (CNPEM), Zip Code 13083-100 Campinas, Sao Paulo, Brazil;3. Brazilian Biorenewables National Laboratory (LNBR), Brazilian Center for Research in Energy and Materials (CNPEM), Zip Code 13083-100 Campinas, Sao Paulo, Brazil;4. Dalton Mass Spectrometry Laboratory, Institute of Chemistry, University of Campinas, Zip Code 13083-970 Campinas, Sao Paulo, Brazil;5. Laboratory of Membrane Trafficking Mechanisms, Department of Integrative Life Sciences, Graduate School of Life Sciences, Tohoku University, Aobayama, Aoba-ku, Sendai, Miyagi 980-8578, Japan;6. Department of Physiological Chemistry, Faculty of Medicine and Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan;7. Institute of Chemistry, University of Campinas, Zip Code 13083-970 Campinas, Sao Paulo, Brazil;1. Department of Computer Science and Engineering, Indian Institute of Technology (BHU), Varanasi 221005, India;2. Department of Electronics and Communication Engineering, VNR VJIET, Hyderabad, India;1. Department of Electronics and Computer Engineering, Ngee Ann Polytechnic, Clementi 599489, Singapore;2. Department of Biomedical Engineering, School of Science and Technology, SIM University, Clementi 599491, Singapore;3. Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur 50603, Malaysia;4. Iwate Prefectural University (IPU), Faculty of Software and Information Science, Iwate, Japan;5. Department of Psychiatry, Research Associate, St. John''s Research Institute, Bangalore 560034, India;6. Department of Instrumentation and Control Engineering, Manipal Institute of Technology, Manipal 576104, India;7. Department of Electronics and Telecommunications, Politecnico di Torino, Italy;8. Department of Biomedical Imaging, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia |
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| Abstract: | TT virus (TTV) is a human circovirus with a single-stranded, circular DNA genome of 3.8 kb. A method was developed to detect IgM antibodies to TTV as a serological marker for the diagnosis of acute TTV infection. IgM antibodies in test sera were captured by a monoclonal antibody against IgM/μ on a solid support followed by binding of IgM with TTV derived from fecal extract of a TTV carrier. The presence of IgM-specific TTV particles was determined by polymerase chain reaction (PCR) using nucleic acids extracted from the solid support. Anti-TTV IgM was detected in sera from two patients with non-A to G post-transfusion hepatitis, who were positive for TTV DNA during weeks 10–21 and 12–17, respectively, following transfusion. The anti-TTV IgM was detectable after alanine transaminase levels were elevated and TTV DNA was detectable in the patients. The duration of the anti-TTV IgM was short-lived compared with anti-TTV IgG. Anti-TTV IgM was not detected in sera from any of 36 healthy individuals, with no detectable anti-TTV IgG or TTV DNA in their serum. These results indicate that anti-TTV IgM antibodies would be a useful marker to detect acute TTV infection. |
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