藏灵菇发酵乳对D-半乳糖衰老小鼠脑组织抗衰老机制研究

目的：通过对小鼠腹腔注射D—gal制造衰老模型小鼠。方法：用藏灵菇发酵乳对小鼠进行灌胃处理，采用黄嘌呤氧化酶法，TBA反应比色法，测定小鼠脑组织中总超氧化物歧化酶（T—SOD），谷胱甘肽过氧化物酶（GSH—Px）活性，丙二醛（MDA）的含量变化，进一步研究藏灵菇发酵乳的抗氧化抗衰老作用及其机理。结果：发现藏灵菇发酵乳能极显著提高D-半乳糖所致衰老小鼠脑组织T—SoD活性，显著提高GSH-Px活性，极显著降低MDA的含量。结论：证明藏灵菇发酵乳具有显著清除衰老小鼠脑组织氧自由基能力，提高抗氧化酶活性，抑制小鼠脑自由基从而发挥抗衰老作用。

Study on the anti--aging mechanism of Tibetan Kefir fermented milk on D-- galactose aging mice brain tissue

Objective： Through the establishment of D--gal induced subacute senile mouse model. Methats： Given Tibetan Kefir fermented milk gastric lavage treatment, to determine the total superoxide dismutase（T-- SOD）, glutathione peroxidase （GSH-- Px） activity, malondialdehyde （MDA） content changes in brain tissue of mice, and further study antioxidant effect and its mechanism of Tibetan Kefir fermentation milk. Results： The results showed that Tibetan Kefir of fermented milk can significantly improve D-- galactose induced aging mice brain tissue T--SOD activity, significantly increase the GSH--Px activity, decreased significantly the content of MDA. Conclusion： It has been proved that Tibetan Kefir fermented milk has a significant effect on scaveng- ing oxygen free radicals in brain tissue of aging mice, improving the antioxidant enzyme activity, and inhibiting mouse brain free radical so as to play the anti--aging role.