| HPLC法测定生脉注射液中5-羟甲基糠醛的含量 |
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| 引用本文： | 徐世霞.HPLC法测定生脉注射液中5-羟甲基糠醛的含量[J].中国民族民间医药杂志,2011(14):36-37. |
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| 作者姓名： | 徐世霞 |
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| 作者单位： | 广西北海食品药品检验所,广西北海536000 |
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| 摘 要： | 目的：建立生脉注射液中5-羟甲基糠醛的含量测定方法。方法：采用高效液相色谱法，色谱柱为Phenomenex Gemini C18（250mm×4．6mm，5μm），流动相为甲醇-水（5：95），检测波长为284nm，流速为1．0ml·ml^-1。结果：5-羟甲基糠醛的线性范围为1．981～118．860Wg（r=0．9998）；浓度范围内进样量与峰面积线性关系良好。平均回收率分别为101．28％（RSD=1．12％）（n=6）。结论：所建方法简单、准确，可用于生脉注射液中5-羟甲基糠醛的含量测定。
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| 关 键 词： | 生脉注射液 5-羟甲基糠醛 高效液相色谱法 |
Determination of 5 - Hydroxymethyl - furfural in Shenmai Injection by HP LC |
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| Xu Shixia.Determination of 5 - Hydroxymethyl - furfural in Shenmai Injection by HP LC[J].Chinese Journal of Ethnomedicine and Ethnopharmacy,2011(14):36-37. |
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| Authors: | Xu Shixia |
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| Institution: | Xu Shixia (Guangxi Beihai Institute for Food and Drug Contro 1 , Beihai 536000 , China) |
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| Abstract: | Objective： To establish a method for determination of 5 - Hydmxymethyl - furfural in Shenmai Injection. Method： The separation was performed on Phenomenex Gemini C18 （ 250mm × 4. 6mm, 5μm ） columm with detection wave length of 284 nm. The mobile phase consisted of methanol - water （5：95 ） with flow rate of 1.0 ml · ml^-1. Result： The linear ranges of 5 - Hydroxym- ethyl - furfural were at 1.981 - 118. 860 p,g （ r = 0. 9998 ）, the average recoveries （ n = 6 ） were 101.28 % （ RSD = 1.12% ） o Conclusion： This method is simple, accurate and suitable for the determination of 5 - Hydroxymethyl - furfural in Shenmai Injection. |
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| Keywords: | Shenmai Injection 5 - Hydroxymethyl - furfural HPLC |
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