| 醋酸铅体外诱发人外周血淋巴细胞HPRT基因位点突变的研究 |
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| 引用本文： | 薛莲,周建华,时锡金.醋酸铅体外诱发人外周血淋巴细胞HPRT基因位点突变的研究[J].工业卫生与职业病,2004,30(2):80-82. |
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| 作者姓名： | 薛莲 周建华 时锡金 |
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| 作者单位： | 苏州大学劳动卫生与环境卫生教研室,江苏,苏州,215007;苏州大学劳动卫生与环境卫生教研室,江苏,苏州,215007;苏州大学劳动卫生与环境卫生教研室,江苏,苏州,215007 |
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| 基金项目： | 江苏省自然科学基金社会发展项目 (BS2 0 0 10 5 0 ) |
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| 摘 要： | 目的 了解醋酸铅对人外周血淋巴细胞HPRT基因位点的影响，以探讨铅的致突变性。方法 采用多核细胞法体外研究醋酸铅对人外周血淋巴细胞HPRT基因位点的影响及突变频率。结果 0．25mmol/L醋酸铅即可诱发人外周血淋巴细胞HPRT基因位点突变，且突变频率随着染毒浓度的增加而升高，突变频率Y(10^-3)与醋酸铅浓度C(mmol/L)之间存在一定的剂量-效应关系，直线方程分别为：Y=1．0566 0．1661C(r=0．9582)和Y=1．0762 0．1987C(r=0．9434)。结论 在一定条件下，醋酸铅对人外周血淋巴细胞HPRT基因位点及突变频率有影响，HPRT基因突变频率可望成为接触环境致突变物人群检测的敏感指标。
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| 关 键 词： | 醋酸铅 HPRT基因 突变频率 多核细胞法 |
| 文章编号： | 1000-7164(2004)02-0080-03 |
Study on Frequency of HPRT Locus Mutation in Human Peripheral Lymphocytes Induced by PbAc2 in vitro |
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| XUE Lian,ZHOU Jian hua,SHI Xi jin.Study on Frequency of HPRT Locus Mutation in Human Peripheral Lymphocytes Induced by PbAc2 in vitro[J].Industrial Health and Occupational Diseases,2004,30(2):80-82. |
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| Authors: | XUE Lian ZHOU Jian hua SHI Xi jin |
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| Abstract: | Objective To study the mutational effect of PbAc 2 on HPRT gene of human peripheral lymphocytes in vitro.Methods A simple and sensitive method,cytokinesis block(CB) assay was used to detect the frequency of HPRT locus mutation in vitro induced by PbAc 2 and its dose effect relationship.Results The test showed that PbAc 2 could induce HPRT locus mutation in human lymphocyte and there existed a linear relationship between the concentration of the heavy metal saline and the mutation frequency(MF) of HPRT gene.Conclusions PbAc 2 could result in HPRT locus mutation in lymphocytes under this experimental conditions.It is expected that this assay might be used to study the environmental exposed population in future. |
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| Keywords: | PbAc 2 HPRT gene Mutation frequency(MF) Cytokinesis Block(CB) assay |
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