重组海葵溶细胞素及平颏海蛇磷脂酶A2对血管外膜成纤维细胞增殖的影响

目的 观察重组海葵溶细胞素(rSrc)及平颏海蛇磷脂酶A₂(rPLA₂)对成纤维细胞增殖的影响。方法 体外培养成纤维细胞,应用不同浓度的rSrc和rPLA₂分别进行作用并设立对照进行比较,采用非放射性的MTS/PES法确定成纤维细胞的增殖状态。结果 各组细胞增殖率分别为:对照组0.840±0.061、rSrc 100 μg/ml组0.263±0.044、rSrc 10 μg/ml组0.418±0.054、rSrc 1 μg/ml组0.605±0.063、rSrc 100 ng/ml组0.772±0.054、rSrc 10 ng/ml组0.906±0.072、rPLA₂ 100 μg/ml组0.498±0.076、rPLA₂ 10 μg/ml组0.937±0.112、rPLA₂ 1 μg/ml组0.978±0.145。统计分析显示,低至1 μg/ml的rSrc仍能明显抑制大鼠成纤维细胞的增殖(P<0.01),并呈现剂量依赖性(P<0.05)。rPLA₂ 100 μg/ml组与对照组相比细胞增殖率有显著差异(P<0.05);rPLA₂ 10 μg/ml和rPLA₂ 1 μg/ml组与对照组相比均无显著差异(P>0.05)。结论 rSrc和rPLA₂分别对血管外膜成纤维细胞增殖有一定的抑制作用。

Effects of recombinant Sagartia rosea cytolysin and Lapemis hardwickii phospholipase A2 on adventitial fibroblasts proliferation

Objective To observe the effects of recombinant Sagartia rosea cytolysin (rSrc) and Lapemis hardwickii phospholipase A₂(rPLA₂) on adventitial fibroblasts proliferation. Methods NIH-3T3 cells were cultured and treated with rSrc and rPLA₂ at different concentrations for observation of cell proliferation using non-radioactive MTS/PES assay in comparison with the control group. Results The ratio of cell proliferation was 0.840±0.061 in the control group, and was 0.263±0.044, 0.418±0.054, 0.605±0.063, 0.772±0.054 and 0.906±0.072 in rSrc groups corresponding to rSrc concentrations of 100, 10, 1 μg/ml and 100, 10 ng/ml respectively. rSrc was found to significantly inhibit fibroblast proliferation in a dose-dependent manner when the concentration used was above 1 μg/ml (P<0.05), as compared with the control group (P<0.05). The ratio of cell proliferation was 0.498±0.076, 0.937±0.112 and 0.978±0.145 in rPLA₂ groups corresponding to rPLA₂ concentrations of 100, 10, 1 μg/ml respectively, indicating that rPLA₂ also significantly inhibited fibroblast proliferation at the concentration of 100 μg/ml (P<0.05). Conclusion rSrc and rPLA₂ can both significantly inhibit adventitial fibroblast proliferation.