Distribution of a morphogenic peptide activating the head growth of hydra in rat organs following intravascular administration of3H-labeled peptide |
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| Authors: | E I Kalenikova V A Tishchenko A Yu Rubina A S Sokolov Yu A Blidchenko T V Kuznetsova Zh D Bespalova |
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| Institution: | (1) Cardiology Research Center, Russian Academy of Medical Sciences, Moscow;(2) Department of Basic Medicine, M. V. Lomonosov State University, Moscow |
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| Abstract: | The organ distribution of radioactivity following intravascular bolus injection of3H-Lys-head growth activator in rats was studied. Two minutes after injection the renal level of radioactivity exceeded the
blood level 7-fold; 80% of the total activity was bound with the blood cell membranes. An analysis of chemical derivatives
of the labeled peptide in the plasma by means of reverse-phase high-performance liquid chromatography revealed the presence
of several groups of radioactive metabolites with different hydrophilic properties. High-performance liquid chromatography
of blood extracts obtained from samples taken 0.5, 1, 1.5, 2, 31, and 60 min after injection showed the transformation of
initially hydrophobic head growth activator into more hydrophilic fragments. The3H-Lys-head growth activator-associated radioactivity could be reliably detected in the blood onl during the first two minutes
after injection. The half-period of blood-to-organ distribution of3H-labeled head growth activator lasted less than 30 seconds.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 118, N
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11, pp. 466–469, November, 1994
Presented by E. I. Chazov, Member of the Russian Academy of Medical Sciences |
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| Keywords: | hydra head growth activator tissue and organ distribution pharmacokinetics metabolism |
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