蟾蜍灵诱导人白血病HL60细胞凋亡的初步研究

目的 :探讨中药蟾蜍灵诱导白血病HL60细胞凋亡作用。方法 :应用台盼蓝染色法测定HL6 0细胞的生长曲线 ,应用相差显微镜、电子显微镜和DNA琼脂糖凝胶电泳等技术分析细胞凋亡。结果 :蟾蜍灵与HL60细胞共育时 ,能明显抑制细胞生长 ,细胞呈典型的凋亡形态学改变 :细胞皱缩 ,染色质凝集 ,沿核膜内侧分布 ,呈新月形 ,可见由膜包被细胞内容物的凋亡小体的产生 ;细胞DNA裂解成 180～200bp及其倍数的片段 ,电泳得到特有的梯形条带。结论 :蟾蜍灵诱导细胞凋亡是其抗肿瘤的作用机制之一。

Preliminary Study on Apoptosis of Human Leukemic Cells Induced by Bufalin

OBJECTIVE: To study the mechanism of inhibiting actions of bufalin on human leukemic cells (HL60). METHODS: HL60 cells were incubated with bufalin at different concentrations and growth inhibition was analyzed by trypan-blue staining. Cell apoptosis was evaluated by phase contrast microscopy, transmission eletron microscopy and agarose gel electrophoresis. RESULTS: Bufalin remarkably inhibited the growth of HL60 cells. Apoptosis of HL60 cells could be effectively induced by bufalin at concentration of 0.01 mumol.L-1 or higher, showing the apoptotic changes in morphology, including cell shrinkage, chromatin agglutination and formation of apoptotic corpuscula; DNA cleavage was observed with agarose gel electrophoresis. CONCLUSION: Bufalin can effectively induce apoptosis of HL60 cells, which is possibly one of the mechanisms for anti-cancer of bufalin.