自体骨髓间充质干细胞复合去抗原牛松质骨载体修复兔桡骨节段性骨缺损

目的：探讨以自体骨髓问充质干细胞（MSCs）复合去抗原牛松质骨载体（BCB）修复兔桡骨节段性骨缺损的效果。方法：抽取20只新西兰大白兔的骨髓并分离、培养、扩增和建立诱导MSCs，将其和BCB复合。手术建立兔双桡骨干15mm骨骨膜缺损的动物模型，实验侧植入MSCs／BCB，对照侧植入BCB，通过X线、组织学检查等手段观察不同时相的骨缺损修复情况。结果：在第12周时，实验侧骨缺损得到骨性修复，对照侧则未能修复。结论：MSCs在体内能成骨，MSCs／BCB能有效地修复节段性骨缺损。

Repairing Radial Segmental Defects in Rabbits with Mesenchymal Stem Cells and Antigen-extracited Bovine Cancellous Bonecarrier

s Objective:To study the efficacy of antigen-extracited bovine cancellous bonecarrier(BCB) loaded with autologous bone marrow mesenchymal stem cells(MSCs) on the healing of rabbit radial segmental defects.Methods:Autologous MSCs were isolated in vitro,then implanted into the BCB.An experimental model of 15mm bone defect was made in the bilateral radius of 20 rabbits.In the experimental group,the MSCs/BCB was implanted into one radial segmental defect and the BCB was implanted into the other one.After the operation,serial radiographic examination and histological analysis were undertaken.Results:In the experimental group,the bone defects healed by 12 weeks,but in the control group,they did not.Conclusion:MSCs can form bone in vivo and MSCs/BCB can repair segmental defects.