壳三糖苷酶活性及其基因多态性与冠心病的关系

目的：通过对血清中壳三糖苷酶活挫及其基因多态性的研究，探讨其与冠心病和冠状动脉狭窄程度之间的关系。方法：对126例冠状动脉造影术（coronary angiography，CAG）阳性患者的血清壳三糖苷酶活性、C-反应蛋白（cRP）及白介素-6（IL-6）水平进行分析，并根据CAG结果将其按冠状动脉狭窄程度及不同狭窄支数分组。与对照组分析比较其临床意义，同时对壳三糖苷酶基因第10号外显子的24个碱基对重复序列突变进行研究。结果：冠心病组血清壳三糖苷酶活性、CRP及IL-6水平均较对照组显著升高，同时多支狭窄的病人其壳三糖苷酶活性大于1支狭窄的病人（P〈0．05），冠脉狭窄程度不同者差异也存在显著性（P〈0．05），但CRP、IL-6在不同分组中无明显差异。壳三糖苷酶第10号外显子24bp核苷酸缺失多态性分析结果显示，冠心病组和对照组之间的基因型频率和等位基因频率差异均无显著性（P〉0．05）。结论：血清壳三糖苷酶活性与冠状动脉病度密切相关，有望作为动脉粥样硬化进程的标志物。未发现对照组与冠心病组之间壳三糖苷酶基因型（NN、ND、DD）和等位基因（N、D）的频率差异存在显著性，提示该基因变异不一定是中国人群冠心病发病的易感因素。

A study on the relationship between chitotriosidase activity and its genetic polymorphism and coronary heart disease

Objective To investigate the relationship between chitotriosidase and coronary heart disease along with the stenotic extent of coronary artery by detecting serum chitotriosidase activity and its genetic polymorphism. Methods An analysis on serum chitotriosidase,CRP and IL-6 was conducted in 126 patients who had abnormal coronary angiography (CAG) findings. The patients were then divided into different groups according to the stenotic extent of coronary artery and the number of stenotic branches. The data was analyzed and compared with that from the control group. We also assayed repeat mutations of sequence in 24-base pairs at exon 10 of chitotriosidase gene. Results The levels of serum chitotriosidase, CRP and IL-6 in the CHD group were much higher than those in the control group, and the chitotriosidase activity in patients with multiple branch stenosis was higher than those in those with single stenosis(P < 0.05). A significant difference in chitotriosidase activity was observed in patients with different artery stenostic levels(P < 0.05) but no obvious differences in CRP and IL-6 were among the different groups. The analysis on polymorphism of nucleotide deletion in 24 bp at exon 10 of chitotriosidase gene showed that there were no differences in the frequency of genotypes and alleles between the studied groups (P > 0.05). Conclusions The activity of chitotriosidase is closely related to the severity of coronary artery stenosis and may be used as a marker in the progression of atherosclerosis. No significant differences in the frequency of chitotriosidase genotypes (NN, ND and DD) and alleles (N and D) were found between the patients with CHD and the controls, suggesting the gene mutation of chitotriosidase is not a definite risk factor for CHD in the Chinese population.