泰州地区流感病毒病原学检测分析

目的研究泰州地区近期流感病毒的流行情况，为制定防治决策提供实验室依据。方法在流感监测点和爆发点采集流感样病例鼻咽拭子，用狗肾传代细胞培养技术对采集标本进行病毒分离培养，用“O”型人血红细胞检测培养物的血凝活性，血凝试验阳性且滴度大于1：8的毒株送江苏省疾病预防控制中心确认和分型。结果从泰州145份流感样病例鼻咽拭子标本中成功分离出21株流感病毒，分离率为14．5％。经江苏省疾控中心鉴定H1N1型9株，B型12株。结论泰州市流感流行毒株型别与江苏省内其他监测点相符；病毒分离培养是流感实验室监测中敏感且准确的方法之一。

Virological and pathogenic detection of influenza virus in Taizhou area

Objective To investigate the prevalence of influenza viruses in Taizhou City at present and provide laboratory evidence for making control measures.Methods Nasopharyngeal swab samples were collected from flu-like patients in monitoring sites and the sites of outbreak for isolation of flu viruses with MDCK cells.Hemagglutination test was conducted with blood group O human RBC.The viral strains positive in hemagglutination tests and with the titre of >1:8 would be confirmed and differentiated by Jiangshu Provincial Center for Disease Control and Prevetion.Results 21 virulent strains including that 9 subtype H_1N_1 and 12 type B,were isolated from 145 nasopharyngeal swab samples,with an isolating rate of 14.5%.Conclusion The types of prevalent strains of influenza in Taizhou are similar with that isolated in other monitoring sites in Jiangshu.Laboratory culture and isolation are the highly sensitive and accurate techniques for laboratory monitoring of influenza viruses.