电针改善慢性疲劳大鼠疲劳状态及痛阈的下丘脑CRH机制研究

目的:探讨电针对慢性疲劳大鼠疲劳状态、痛阈的影响及其中枢机制。方法:96只SD大鼠随机分为空白组、模型组、模针组、侧脑室假注射组(假注射组)、侧脑室注射促肾上腺皮质激素释放因子组(CRF组)、侧脑室注射CRF电针组(CRF电针组)、侧脑室注射CRFA组(CRFA组)、侧脑室注射CRFA电针组(CRFA电针组),除空白组外,采用慢性束缚复合强制性冷水游泳复制慢性疲劳模型。假注射组行侧脑室假注射,CRF组、CRF电针组行侧脑室CRF注射,CRFA组、CRFA电针组行侧脑室CRF拮抗剂注射。模针组、CRF电针组、CRFA电针组电针"肾俞"、"足三里"治疗。分别在造模前、造模后、治疗后进行力竭游泳实验、水迷宫实验、热板测痛实验检测疲劳状态及痛阈。结果:造模后,造模各组力竭游泳时间较空白组明显缩短(P0.05),水迷宫时间较空白组明显延长(P0.05),热痛时间较空白组明显缩短(P0.05)。治疗后与模型组比较,模针组、CRF电针组力竭游泳时间明显延长(P0.05),水迷宫时间明显缩短(P0.05),热痛时间明显延长(P0.05);CRFA电针组力竭游泳时间、水迷宫时间、热痛时间较模型组均无明显变化。结论:电针肾俞、足三里可改善慢性疲劳大鼠体力和脑力疲劳以及易感疼痛情况,下丘脑CRH是电针治疗作用的关键物质。

Mechanism of Electroacupuncture Improvement to the Fatigue and Pain Threshold in Chronic Fatigue Rats

Objective： To explore the effect of electroacupuncture of behavior changes in chronic fatigue rats and the mechanism. Methods： 96 SD rats were randomly divided into normal group, model group, model electroacupuncture group （EA group）, sham injection group （SI group）, CRF injection group （CRF group ）, CRF injection and electroacupuncture group （CRFEA group）, CRFA injection group （CRFA group）, CRFA injection and electroacupuncture group （CRFAEA group） . All the rats except the normal group rats were restrainted and swam in cool water. The rats in the CRF group, CRFEA group, CRFA group and CRFAEA group were intracerebroventricular injected. The rats in the SI group were sham intracerebroventricular injected. The rats in the EA group, CRFEA group and CRFAEA group were treated by electroacupuncture on BL23, ST36. The exhaustive swimming time, Finding the platform time of Morris water maze test and reaction time to pain were tested. Results： After modeling, comparison withnomlal group, tile exhaustive swimming time and reaction time to pain in the other groups were significantly shorter （ P 〈 0.05 ） , and the finding the platform time were significantly longer （ P 〈 0. 05 ） . After treatment, comparison with model group, the exhaustive swimming time and reaction time to pain in EA groups and CRFEA group were significantly longer （ P 〈 0. 05 ） , and the finding the platform time were significantly shorter （ P 〈 0. 05 ） . Conclusion： Electroacupuneture can alleviate physical and mental fatigue and improve pain threshold in chronic fatigue rats. The CRH in hypothalamus is important to eleetroaeupuncture.