| 利用α—factor启动子在酵母中表达乙型肝炎表面抗原基因 |
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| 引用本文： | 林哈娜 张福徽. 利用α—factor启动子在酵母中表达乙型肝炎表面抗原基因[J]. 中国医学科学院学报, 1989, 11(3): 170-174 |
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| 作者姓名： | 林哈娜 张福徽 |
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| 作者单位： | 中国医学科学院基础医学研究所 北京(林哈娜，张福徽，王年，刘承斌，欢玲君，饶春明)，中国医学科学院基础医学研究所 北京(梁植权) |
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| 摘 要： | 利用酵母α-factor分泌型启动子构建大肠杆菌—酵母菌穿梭质粒。将乙型肝炎表面抗原基因(HBsAg基因)经过调正读码框架使其准确地和表达载体相连接,最终在酵母中成功地表达HBsAg蛋白并分泌到细胞外。
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| 关 键 词： | 基因 穿梭质粒 读码框架 克隆株 |
Expression of gene encoding HBsAg in yeast cell with its alpha-factor promoter |
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| Lin Hana. Expression of gene encoding HBsAg in yeast cell with its alpha-factor promoter[J]. Acta Academiae Medicinae Sinicae, 1989, 11(3): 170-174 |
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| Authors: | Lin Hana |
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| Abstract: | Construction of an E. coli-yeast shuttle plasmid was reported with yeast alpha-factor as promoter of synthesis and secretion of mature foreign proteins by yeast cells (Saccharomyces cerevisiae). The HBsAg gene was successfully recombined into the plasmid with its frame precisely adjusted, thus resulting in extracellular secretion of HBsAg protein by the yeast cells. |
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| Keywords: | gene shuttle plasmid frame adjustment cloned strain |
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