生物素标记DNA探针测定人性别

白细胞或胎儿绒毛组织染色体DNA经限制酶Hae (?)消化后,用光生物素(photo-biotin)标记人Y染色体特异的3.4 kb DNA片段作探针,进行Southern印迹杂交,并以生物素结合蛋白-碱性磷酸酶体系显示生物素探针杂交信号。结果表明,0.1μg男性DNA可显示清晰的信号,而5μg女性DNA则无信号出现;平行试验证明生物素探针测定人性别的结果与32P标记探针完全一致。提示生物素探针可用于X连锁遗传病、性异常,运动员体检,法医学及异性别骨髓移植方面的性别分析。

Biotinylated Y Chromosome Specific Probe for Human Sexing

Human chromosome DNA from WBC or fetus chorion samples were digested with Hae III and hybridized with biotinylated Y chromosome specific probe by Southern blotting, and hybridization signals were developed by the ABC (Avidin-biotin-alkaline phosphatase complex) system. The hybridization signal for 0.1 microgram of male DNA could be detected clearly, while the signal for even 5 micrograms of female DNA could not. Parallel tests showed that the sexing results using 32P-labeled and biotinylated Y probe were identical. This suggests that the biotinylated Y probe can be applied to the determination of X-linked genetic diseases and sex abnormality, forensic analysis, sex determination of sportsmen and women, heterosexual transplantation of bone marrow, etc. It could become a convenient means for genetic diagnosis.