| 用BPV转化灶做选择标记表达HBsAg的研究 |
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| 引用本文： | 温晓燕.用BPV转化灶做选择标记表达HBsAg的研究[J].中国医学科学院学报,1989,11(5):373-375. |
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| 作者姓名： | 温晓燕 |
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| 作者单位： | 中国预防医学科学院病毒学研究所 北京 |
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| 摘 要： | 作者构建了pBMTHBR2质粒,此质粒含有鼠MT启动子、完整的HBsAg基因(preS和S基因)、SV_(40)的拼接信号和完整的BPV基因组。用磷酸钙沉淀技术把此质粒导入鼠C_(127)细胞内,用BPV转化灶做标记,获得了转化细胞克隆。实验表明,57%的克隆细胞系均能分泌HBsAg。
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| 关 键 词： | HBsAg 基因表达 牛乳头瘤病毒载体 |
Using BPV Transforming Foci as Selective Markers to Express HBsAg |
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| Wen Xiaoyan,et al.Using BPV Transforming Foci as Selective Markers to Express HBsAg[J].Acta Academiae Medicinae Sinicae,1989,11(5):373-375. |
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| Authors: | Wen Xiaoyan |
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| Abstract: | We constructed a plasmid pBMTHBR2 containing mouse MT promoter, entire HBsAg gene (preS and S gene), splicing signal from SV40 and complete BPV genome. Using calcium phosphate precipitation technique to transform C127 Cells and using BPV transforming foci as selective markers, we obtained transformed cell clones. The experiment shows that 57% of the cloned cell lines can secrete HBsAg continuously. |
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| Keywords: | HBsAg gene expression bovine papilloma virus(BPV) |
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