| 人乳头瘤病毒DNA在宫颈癌组织中的整合状态 |
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| 引用本文： | 韩日才 司静懿. 人乳头瘤病毒DNA在宫颈癌组织中的整合状态[J]. 中国医学科学院学报, 1989, 11(3): 234-236 |
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| 作者姓名： | 韩日才 司静懿 |
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| 作者单位： | 中国医学科学院基础医学研究所 北京(韩日才，司静懿，李昆，王申五，张卫)，中国医学科学院基础医学研究所 北京(宋国兴) |
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| 摘 要： | 人乳头瘤病毒(HPV)在宫颈癌组织中是否整合,各家报道不一。多数研究证实良性病变中的HPV以游离状态存在,而恶性病变中的HPV DNA则已与宿主细胞基因整合。本实验从HPV 16杂交阳性病例中随机抽取5例标本。应用四种方法研究HPV 16在宫颈癌组织中的整合状态,旨在阐述HPV的致癌机理提供实验依据.
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| 关 键 词： | 人乳头瘤病毒 宫颈癌 致癌机理 |
Studies on the physical state of human papillomavirus 16 DNA in biopsies of patients with cervical carcinoma |
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| R C Han. Studies on the physical state of human papillomavirus 16 DNA in biopsies of patients with cervical carcinoma[J]. Acta Academiae Medicinae Sinicae, 1989, 11(3): 234-236 |
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| Authors: | R C Han |
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| Abstract: | Five biopsies from cervical cancer containing human papillomavirus (HPV) 16 DNA sequences confirmed by dot blot analysis were used to study the physical state of HPV 16 DNA in cervical cancer. Southern blots prepared after: 1) sample DNA was cleaved with restriction enzymes which do not digest HPV 16;2) sample DNA was cleaved with restriction enzymes (pstI) capable of digesting HPV 16 DNA; 3) sample DNA was not digested with restriction enzymes; and 4) two-dimensional agarose gel electrophoresis was performed on sample DNA digested with enzymes which do not cleave HPV 16 DNA. It was shown that HPV 16 DNA was integrated into the cellular genome. |
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