外科原位移植人前列腺癌自发转移模型的建立

目的 建立人前列腺癌裸鼠自发转移模型，观察其生长和转移的生物学特性。方法 利用外科原位移植的，取材于人前列腺癌细胞系ＰＣ－３Ｍ裸鼠皮下移植瘤，保持肿瘤组织结构的完整性，通过显微外科手术植于裸鼠前列腺两腹侧叶之间，濒死时处死。结果 动物平均３０ｄ衰竭，处死，原位移植成功率１００％，主动脉地转移发生率１００％，较ＰＣ－３Ｍ肿瘤细胞原位注及ＰＣ－３外科原位移植主动脉旁淋巴结转移发生率更高。结论 肿瘤细胞

Metastatic Model for Human Prostate Cancer Using Surgical Orthotopic Implantation in Nude Mice

Objective Establishment of an animal model of human prostate cancer demonstrating spontaneous meastasis by surgical orthotopic implantation to understand the disease and to formulate treatment strategy.Methods We implant histologically intact tumor tissues orthotopically in immunodeficient mice.In this study intact tissue of the human prostate cancer cell line PC-3M,harvested from a subcutaneous tumor in a nude mouse,was implanted to the ventral lateral lobes of the prostate gland in a serise of nude mice.Mice were sacrificed when found moribund,and autopsy and histology were performed subsequently.Results In contrast to orthotopic injectioin of PC-3M and surgical orthotopic implantation of PC-3,surgical orthotopic implantation of PC-3M can cause higher frequency of periaortic lymph node metastasis noted upon histological examination.Conclusion Stromal tissue as well as metastatic cells played a critical role in tumor growth an progression. [