Bcr/abl+ autologous dendritic cells for vaccination in chronic myeloid leukemia |
| |
Authors: | Westermann J Kopp J Körner I Richter G Qin Z Blankenstein T Dörken B Pezzutto A |
| |
Affiliation: | Dept of Hematology, Oncology and Tumorimmunology, Robert R?ssle Klinik, Charité, Humboldt University, Berlin, Germany. |
| |
Abstract: | In chronic myeloid leukemia (CML) ex vivo generated DC are characterized by constitutive expression of bcr/abl and possibly other yet undefined leukemia-associated antigens, since these DC share a common progeny with leukemic cells. Induction of anti-leukemic T cell responses has been described in vitro. For a phase I vaccination study, autologous bcr/abl+ DC are generated under GMP conditions mainly from monocyte precursors in chronic phase CML patients. Lin-, CD80+, CD86+, CD83+, DR+ DC could be generated in sufficient numbers for s.c. vaccination with 1 x 10(6)-5 x 10(7) DC. Using monocyte precursors, the yield of DC per seeded PBMC was in the range of 1-6%. Furthermore, we could demonstrate in vitro that the T cell stimulatory ability of CD34+-derived DC can be augmented by a factor 2-3 by retroviral transduction with a gene coding for interleukin-7. DC-based vaccination strategies are a promising clinical approach, particularly as postremission immunotherapy in the setting of autologous stem cell transplantation. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|